摘要
目的:探讨骨科创伤后感染患者金黄色葡萄球菌耐药基因及毒力基因的分布情况。方法:收集40株骨科创伤后金黄色葡萄球菌感染患者标本,采用聚合酶链式反应(PCR)法对菌株耐药基因(mecA)、黏附素基因(bbp、clfA、clfB、can、ebpS、fnbA、sdrC、sdrD、sdrE、icaA、map)、溶血毒素基因(hla、hlb、hld、hlg)、杀白细胞素基因(lukE、lukM、pvl)、侵袭毒素基因(splB、ssp)进行检测,比较不同感染程度及不同感染控制情况患者菌株中毒力基因分布情况。结果:40株金黄色葡萄球菌中共检出mecA基因耐药株数23株(耐药率57.50%),即耐甲氧西林金黄色葡萄球菌(MRSA)为23株,对甲氧西林敏感的金黄色葡萄球菌(MSSA)为17株。深部感染组患者sdrC基因检出率高于浅表感染组(72.73%vs.33.33%,P<0.05)、ssp基因检出率高于浅表感染组(100.00%vs.66.67%,P<0.05);浅表感染组与深部感染组患者其余基因检出率比较,差异无统计学意义(P>0.05)。无效组患者sdrD基因检出率低于有效组(11.11%vs.61.29%,P<0.05)、pvl基因检出率高于有效组(33.33%vs.3.33%,P<0.05);有效组与无效组患者其余基因检出率比较,差异无统计学意义(P>0.05)。结论:毒力基因sdrC、ssp与骨科创伤后金黄色葡萄球菌感染患者感染程度相关,毒力基因sdrD、pvl与骨科创伤后金黄色葡萄球菌感染患者预后转归(感染控制情况)相关。
Objective:o investigate the distribution of drug resistance genes and virulence genes of Staphylococcus aureus in patients with orthopaedic post-traumatic infection.Methods:40 strains of Staphylococcus aureus isolated from orthopaedic posttraumatic infection patients were selected.Polymerase chain reaction(PCR)was used to detect drug resistance gene(mecA),adhesin gene(bbp,clfA,clfB,can,ebpS,fnbA,sdrC,sdrD,sdrE,icaA,map),hemolytic toxin gene(hla,hlb,hld,hlg),leukocidin gene(lukE,lukM,pvl),invasive toxin gene(splB,ssp),and the distribution of virulence genes in patients with different degrees of infection and different infection control conditions were compared.Results:A total of 23 MECA gene resistant strains(57.50%)were detected in 40 strains of Staphylococcus aureus,23 strains of methicillin resistant Staphylococcusaureus(MRSA)and 17 strains of methicillin sensitive Staphylococcusaureus(MSSA).The detection rate of sdrC gene in deep infection group was higher than that in superficial infection group(72.73%vs.33.33%,P<0.05),and the detection rate of ssp gene was higher than that in superficial infection group(100.00%vs.66.67%,P<0.05).There was no significant difference in the detection rate of other genes between superficial infection group and deep infection group(P>0.05).The detection rate of sdrD gene in the ineffective group was lower than that in the effective group(11.11%vs.61.29%,P<0.05),and the detection rate of pvl gene in the ineffective group was higher than that in the effective group(33.33%vs.3.33%,P<0.05).There was no significant difference in the detection rate of other genes between ineffective group and effective group(P>0.05).Conclusion:The virulence genes sdrC and ssp are correlated with the infection degree of patients with post-traumatic Staphylococcus aureus infection in orthopedics,and the virulence genes sdrD and pvl are correlated with the prognosis(infection control)of patients with post-traumatic Staphylococcus aureus infection in orthopedics.
作者
段俊林
陈日寿
陈伟
叶林强
冯永洪
黄祖辉
刘亮洪
DUAN Jun-lin;CHEN Ri-shou;CHEN Wei;YE Lin-qiang;FENG Yong-hong;HUANG Zu-hui;LIU Liang-hong(Department of Laboratory,Dongguan Hospital of Traditional Chinese Medicine,Dongguan 523000,Guangdong,China;Department of Orthopedics,Dongguan Hospital of Traditional Chinese Medicine,Dongguan 523000,Guangdong,China)
出处
《川北医学院学报》
CAS
2021年第12期1600-1603,共4页
Journal of North Sichuan Medical College
基金
广东省东莞市社会科技发展项目(201950715002755)。
关键词
金黄色葡萄球菌
耐药基因
毒力基因
骨科
创伤后感染
Staphylococcus aureus
Drug resistance gene
Virulence gene
Orthopedics
Post traumatic infection
