摘要
【目的】研究彩色小麦组织培养再生体系,为建立彩色小麦高频遗传转化体系提供理论基础。【方法】以中黑麦6号、CM-Z2和中蓝麦1号3个彩色小麦品种(系)的成熟胚作为外植体材料,在愈伤诱导8、14、20 d分别统计诱导情况;采用IAA+KT、IAA+6-BA生长调节剂组合进行彩色小麦愈伤组织的再分化,比较不同处理愈伤组织出愈率、分化率、成苗率等。【结果】中黑麦6号、CM-Z2、中蓝麦1号外植体在培养14 d时愈伤诱导效果最佳,出愈率在80.54%~95.39%。彩色小麦成熟胚愈伤组织再生能力与基因型、培养基有关,中黑麦6号再生能力最好,分化率在87.68%~97.50%、成苗率在17.26%~61.73%,在含有0.1 mg/L IAA和0.5 mg/L 6-BA、0.5 mg/L IAA和4 mg/L KT的分化培养基中成苗率较高,分别为61.73%、56.25%;其次为CM-Z2,分化率在60.23%~95.45%、成苗率在2.27%~34.09%,在含有0.5 mg/L IAA和4 mg/L KT的分化培养基中成苗率最高,为34.09%。【结论】中黑麦6号是彩色小麦遗传转化的良好材料;0.1 mg/L IAA和0.5 mg/L 6-BA、0.5 mg/L IAA和4 mg/L KT可以作为彩色小麦中黑麦6号和CM-Z2良好的再生培养基。
【Objective】To study the color wheat tissue culture regeneration system and provide a theoretical basis for establishing a high-frequency color wheat genetic transformation system.【Methods】The mature embryos of three color wheat varieties(lines)of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 were used.The induction status was recorded at 8,14,and 20 days after callus induction.Growth regulator IAA+KT and IAA+6-BA combination were used to redifferentiate color wheat callus.The callus rate,differentiation rate and seedling rate of different treatments were evaluated.【Results】The explants of Zhongheimai No.6,CM-Z2 and Zhonglanmai No.1 had the best callus induction effect after cultured for 14 days,with callus rates ranging from 80.54%to 95.39%.The regeneration ability of color wheat mature embryo callus was related to genotype and culture medium.Zhongheimai No.6 had the best regeneration ability,with a differentiation rate of 87.68%-97.50%,and a seedling rate of 17.26%-61.73%.The seedling rate was higher in the differentiation medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT,with a rate of 61.73%and 56.25%respectively.CM-Z2 followed Zhongheimai No.6,had a differentiation rate of 60.23%-95.45%and seedling rate of 2.27%-34.09%.CM-Z2 had the highest seedling rate in the differentiation medium containing 0.5 mg/L IAA and 4 mg/L KT,reaching 34.09%.【Conclusion】Zhongheimai No.6 was an ideal material for color wheat genetic transformation.Regeneration medium containing 0.1 mg/L IAA and 0.5 mg/L 6-BA,0.5 mg/L IAA and 4 mg/L KT were suitable for Zhongheimai No.6 and CM-Z2.
作者
谭一罗
陈凤
张广旭
王康君
郭明明
李晓峰
张梦涵
樊继伟
TAN Yiluo;CHEN Feng;ZHANG Guangxu;WANG Kangjun;GUO Mingming;LI Xiaofeng;ZHANG Menghan;FAN Jiwei(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,China)
出处
《北方农业学报》
2021年第5期25-31,共7页
Journal of Northern Agriculture
基金
江苏省农业科技自主创新资金项目[CX(18)3010]
江苏省农业重大新品种创制项目(PZCZ201706)
连云港市财政专项(QNJJ2101,QNJJ1813)。
关键词
彩色小麦
组织培养
成熟胚
再生体系
Color wheat
Tissue culture
Mature embryo
Regeneration system