摘要
为明确不同马铃薯品种叶片内生细菌多样性及差异,为马铃薯抗逆性机理及内生菌开发研究提供依据。利用LB培养基对云南省4个主栽马铃薯品种叶片内生细菌进行分离培养,根据形态特征和16S rDNA序列对菌株进行分类鉴定。从青薯9号马铃薯叶片中分离获得内生细菌3门8科9属10种,其中葡萄球菌属和微小杆菌属为优势菌属,相对多度均为18.18%;从会-2马铃薯叶片中分离获得内生细菌4门11科11属12种,其中不动杆菌属和假单胞菌属为优势菌属,相对多度均为15.38%;从合作88马铃薯叶片中分离获得内生细菌4门9科10属13种,其中不动杆菌属为优势菌属,相对多度为23.53%;从丽薯6号马铃薯叶片中分离获得内生细菌4门7科8属17种,其中葡萄球菌属为优势菌属,相对多度为22.22%。4个马铃薯品种叶片中可培养细菌共有37种,其中青薯9号、会-2、合作88和丽薯6号马铃薯叶片中独有的内生细菌分别有4,8,4,10种。马铃薯不同品种叶片内生细菌种类组成及优势菌属不同,可为马铃薯叶片内生菌功能研究提供菌种资源。
In order to clarify the diversity and differences of endophytic bacteria in leaves of different potato varieties and provide basis for studying the mechanism of potato stress tolerance and the development of endophytic bacteria.The endophytic bacteria from the leaves of four main potato varieties in Yunnan Province were isolated and cultured on LB medium,all those strains were identified based on the morphological characteristics and the 16S rDNA sequence.There were 10 species of endophytic bacteria belonging to 9 genera,8 families and 3 phyla were isolated from Qingshu No.9 leaves.Staphylococcus and Exiguobacterium were the dominant genera,and the relative abundance of both genera were 18.18%.There were 12 species of endophytic bacteria belonging to 11 genera,11 families and 4 phyla were isolated from Hui-2 leaves.Acinetobacter and Pseudomonas were the dominant genera,and the relative abundance of both genera were 15.38%.There were 13 species of endophytic bacteria belonging to 10 genera,9 families and 4 phyla were isolated from Hezuo No.88 leaves.Acinetobacter were the dominant genera,with a relative abundance of 23.53%.There were 17 species of endophytic bacteria belonging to 8 genera,7 families and 4 phyla were isolated from Lishu No.6 leaves.Staphylococcus were the dominant genera,with a relative abundance of 22.22%.There were 37 species of culturable bacteria were isolated from the leaves of four potato varieties.There were 4,8,4,10 unique endophytic bacteria from the leaves of Qingshu No.9,Hui-2,Hezuo No.88 and Lishu No.6,respectively.The species composition and dominant genera of endophytic bacteria in leaves of different potato varieties were different,the results would provide bacteria resources for studying the function of endophytic bacteria in potato leaves.
作者
杨金睿
王文倩
王玥
姚遥
吉泽
李俊逸
陈斌
肖关丽
YANG Jinrui;WANG Wenqian;WANG Yue;YAO Yao;JI Ze;LI Junyi;CHEN Bin;XIAO Guanli(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)
出处
《华北农学报》
CSCD
北大核心
2021年第6期178-188,共11页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31760519)
云南省教育厅科技项目(2020Y206)。
关键词
马铃薯品种
内生细菌
分子鉴定
马铃薯叶片
可培养细菌
Potato varieties
Endophytic bacteria
Molecular identification
Potato leaves
Culturable bacteria
