摘要
目的:测定复方鲜竹沥液中活性成分2,6-二甲氧基苯酚的含量。方法:采用ACE-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸为流动相梯度洗脱;流速为1.0 mL/min;柱温30℃;检测波长201 nm。结果:进样量在0.043~0.43μg之间的2,6-二甲氧基苯酚具有良好的线性关系(r=0.9999),平均回收率为97.3%,RSD为1.7%(n=6)。结论:本研究建立的方法稳定可靠,可控性强,可用于复方鲜竹沥液2,6-二甲氧基苯酚成分的含量测定。
Objective:Determination of active component 2,6-dimethoxyphenolin fufang xianzhuli ye.Methods:Using ACE-C18 column(4.6 mm×250 mm,5μm),Acetonitrile-0.1%phosphoric acid as mobile phase with gradient elution.The flow rate was 1.0 mL/min;the column temperature was 30℃;the detection wavelength was 201 nm.Results:The injection amount of 2,6-dimethoxyphenol was 0.043~0.43μg(r=0.9999),showing a good linear relationship,the average recovery was 97.3%,and the RSD was 1.7%(n=6).Conclusion:The established method in this study has high sensitivity,good repeatability and strong specificity,and the method can be used for the determination of the content of 2,6-dimethoxyphenol in fufang xianzhuli ye.
作者
黄东
肖小武
周志强
付辉政
许妍
洪挺
HUANG Dong;XIAO Xiaowu;ZHOU Zhiqiang;FU Huizheng;XU Yan;HONG Ting(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang Jiangxi 330029,China)
出处
《药品评价》
CAS
2021年第22期1356-1359,共4页
Drug Evaluation
基金
江西省重点研发项目(20203BBG73062)
江西省药品监督管理局科研项目(2020JS04)。
关键词
禾本科
复方鲜竹沥液
高效液相色谱法
2
6-二甲氧基苯酚
含量测定
Poaceae
Fufang xianzhuli ye
High performance liquid chromatography(HPLC)
2,6-dimethoxyphenol
Content determination
