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Protective Effect of Immaturue Bitter Orange(Citrus aurantium L.)Flavonoids Extracts on PC12 Cell Injury Induced by 6-Hydroxydopamine

枳实黄酮提取物对6-羟基多巴胺所致PC12细胞损伤的保护作用
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摘要 To study the neuro protective effect of flavonoids extracts from immature bitter orange(Citrus aurantium L.),the PC12 cells treated with 6-hydroxydopamine(6-OHDA)were used as the Parkinson’s disease(PD)model.To determine the optimal dose of 6-OHDA for constructing a PD model,PC12 cells were incubated with different concentrations of 6-OHDA for 24 h.After 24 h incubation,PC12 cells of drug groups were added 6-OHDA and different concentrations of flavonoids extracts were measured cell viability by CCK8 for selecting effective concentration of flavonoids extracts;the ROS level was determined using flow cytometry;the levels of MDA,CAT,SOD and GSH-Px were assayed by Colorimetric kit for oxidative stress investigation.Compared with the model group,PC12 cell viability was significantly enhanced(P<0.05),the levels of ROS and MDA were reduced significantly(P<0.05),and the activities of SOD,CAT and GSH-Px were significantly enhanced(P<0.05)in drug groups.In conclusion,immature bitter orange flavonoids extracts could protect PC12 cells against 6-OHDA-induced oxidative stress. 通过6-羟基多巴胺(6-OHDA)诱导PC12细胞损伤模型,研究枳实黄酮提取物的神经保护作用。以不同浓度6-OHDA处理PC12细胞24 h以确定构建帕金森病模型的最适作用剂量,药物组加入6-OHDA和不同浓度枳实黄酮提取物共同孵育细胞24 h。以CCK8法检测细胞存活率,摸索枳实黄酮提取物的有效浓度,采用流式细胞术检测枳实黄酮提取物对6-OHDA损伤的PC12细胞活性氧(ROS)的影响,并以比色法检测其丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)水平。结果显示:与模型组比较,药物组细胞存活率显著升高(P<0.05),ROS含量和MDA水平显著降低,SOD、CAT和GSH-Px活性显著升高(P<0.05)。以上结果表明,枳实黄酮对6-OHDA诱导的PC12细胞氧化损伤有一定的保护作用。
作者 LIANG Zeng-en-ni LI Zhi-jian SHAN Yang 梁曾恩妮;李志坚;单杨(湖南省农业科学院,湖南省农产品加工研究所,湖南长沙410125)
出处 《Agricultural Science & Technology》 CAS 2021年第3期36-41,共6页 农业科学与技术(英文版)
基金 重点领域研发计划(2019NK2041) 湖南省农业科技创新资金项目(2019JG01)。
关键词 Immature bitter orange(Citrus aurantium L.) FLAVONOIDS 6-HYDROXYDOPAMINE PC12 cells 枳实 黄酮 6-羟基多巴胺 PC12细胞
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