摘要
为建立油用牡丹组培快繁体系,本研究以油用牡丹腋芽为外植体,采用不同浓度的消毒剂进行表面消毒,获得无菌苗;将无菌苗分别接种到含有不同激素的MS培养基上,进行侧芽的增殖培养;将获得的再生苗接种到含有不同浓度的生长素培养基上进行生根培养。结果显示:采用15%的安替福民,处理5 min,获得的消毒效果最好;在MS+1.0 mg/L BA+0.5 mg/L IAA+5 mg/L AgNO_(3)培养基上油用牡丹再生芽增殖效果最好;在MS+0.1 mg/L NAA+0.5 g/L AC培养基上再生苗生根效果最好。本试验结果可以为油用牡丹的组培研究奠定基础。
In order to establish the rapid propagation system of Paeonia ostii tissue culture,this study used Paeonia ostii axillary buds as explants and used disinfectant of different concentrations for surface disinfection to obtain bacteria-free seedlings.The results showed that the best disinfection effect was obtained when 15% antihuman was used as disinfectant for 5 min.In MS+1.0 mg/L BA+0.5 mg/L IAA+5 mg/L AgNO_(3) medium,the regeneration bud proliferation effect of Paeonia ostii was the best.The best rooting effect was observed in MS+0.1 mg/L NAA+0.5 g/L AC medium.The results of this experiment can lay a foundation for the study of tissue culture of Paeonia ostia.
作者
任晓琴
杨静慧
刘艳军
张景新
马昕
冯国华
Ren Xiaoqin;YANG Jinghui;Liu Yanjun;Zhang Jingxin;Ma Xin;Feng Guohua(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China;Tianjin Jizhou Forestry Bureau,Tianjin 301900,China;Tianjin Highway Bureau Direct Branch,Tianjin 300074,China)
出处
《天津农学院学报》
CAS
2021年第4期19-22,共4页
Journal of Tianjin Agricultural University
基金
天津市科学技术局项目(18JCTPJC68000)
天津市科技特派员项目(17ZXBFNC00310)。
关键词
油用牡丹
组培
腋芽
MS培养基
Paeonia ostii
tissue culture
axillary bud
MS culture medium
