摘要
目的研究黄芪多糖对野百合碱诱导大鼠肺动脉高压的减缓作用及可能机制。方法将100只SD雄性大鼠随机分为正常对照组、野百合碱组、黄芪多糖低剂量组和黄芪多糖高剂量组。除正常对照组外,其余组大鼠按60 mg/kg单次腹腔注射野百合碱。黄芪多糖低剂量组及高剂量组大鼠在给药后的第2~28天分别按200 mg/kg及400 mg/kg腹腔注射黄芪多糖,每日1次。每组25只大鼠各取15只进行研究,检测各组大鼠平均肺动脉压(mPAP)、右心肥厚指数(RVHI),观察其肺动脉及心肌细胞形态变化,检测其肺组织中白细胞介素17(IL-17)mRNA及蛋白的表达水平。结果与正常对照组比较,野百合碱组及黄芪多糖各剂量组大鼠mPAP、RVHI均显著升高(P<0.01),肺组织中IL-17的mRNA和蛋白表达水平均显著升高(P<0.01),肺动脉及心肌细胞有明显病理改变;与野百合碱组比较,黄芪多糖各剂量组大鼠mPAP、RVHI均显著降低(P<0.01),肺组织中IL-17的mRNA和蛋白表达水平均显著降低(P<0.01),肺动脉及心肌细胞病理变化有改善;与黄芪多糖低剂量组比较,黄芪多糖高剂量组大鼠上述指标水平及病理改变的改善更明显。结论黄芪多糖能降低野百合碱诱导大鼠的肺动脉高压,并改善肺动脉结构及心肌病理改变,推测其机制可能与下调大鼠肺组织中IL-17的表达有关。
OBJECTIVE To study the mitigation effect and its possible mechanism of Astragalus membranaceus polysaccharide on the pulmonary hypertension induced by monocrotaline in rats.METHODS One hundred SD male rats were randomly divided into normal control group,monocrotaline group,A.membranaceus polysaccharide low-dose and high-dose groups.In addition to the normal control group,rats in other groups were injected with monocrotaline by single intraperitoneal injection of 60 mg/kg.On days 2 to 28 after administration,rats in the A.membranaceus polysaccharide low-dose and high-dose groups were intraperitoneally injected with A.membranaceus polysaccharide of 200 mg/kg and 400 mg/kg,respectively,once a day.There were 25 rats in each group,and 15 rats were taken for index detection.The mean pulmonary artery pressure(mPAP)and right heart hypertrophy index(RVHI)were detected,and morphology changes of pulmonary artery and cardiomyocytes were monitored.mRNA and protein expression of IL-17 in their lung tissues were detected.RESULTS Compared with normal control group,mPAP and RVHI of monocrotaline group and A.membranaceus polysaccharide groups were increased significantly(P<0.01);mRNA and protein expression of IL-17 in lung tissues were significantly increased(P<0.01),and there were obvious pathological changes in pulmonary artery and cardiomyocytes.Compared with monocrotaline group,mPAP and RVHI were significantly decreased in A.membranaceus polysaccharide groups(P<0.01),while mRNA and protein expression of IL-17 in lung tissue were decreased significantly(P<0.01);pathological changes in pulmonary artery and cardiomyocytes were improved.Compared with A.membranaceus polysaccharide low-dose group,above indexes and pathological changes were improved significantly in high-dose group.CONCLUSIONS A.membranaceus polysaccharide can reduce monocrotaline-induced pulmonary hypertension,improve pulmonary artery structure and myocardial remodeling in rats,the mechanism of which is presumably related to the down-regulation of IL-17 expression in lung tissue of rats.
作者
张玉坤
何东平
ZHANG Yukun;HE Dongping(Dept.of Respiratory and Critical Care Medicine,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Dept.of Pharmacy,Chongqing Yongchuan District Maternal and Child Health Hospital,Chongqing 402160,China)
出处
《中国药房》
CAS
北大核心
2022年第1期64-68,共5页
China Pharmacy
基金
重庆市永川区科学技术局和重庆医科大学附属永川医院联合资助科技项目(No.Ycstc,2019nb0207)。
关键词
黄芪多糖
野百合碱
肺动脉高压
白细胞介素17
Astragalus membranaceus polysaccharide
monocrotaline
pulmonary hypertension
IL-17
