摘要
为了解雷公藤内生真菌的雷公藤甲素合成能力以及内生真菌间互作对雷公藤甲素合成的影响,本试验从雷公藤中筛选出1株产雷公藤甲素的内生真菌NS-1,鉴定为Fusarium nisikadoi;通过NS-1与其它内生真菌共培养,发现青霉菌属(Penicilliumn)内生真菌NS-8能够显著促进NS-1的雷公藤甲素合成(P<0.05),是对照的1.6倍.以此为基础,通过制备和添加NS-8菌丝体提取物和发酵液诱导子,进一步研究内生真菌NS-8诱导子对NS-1雷公藤甲素合成的影响.结果表明,NS-8发酵液诱导子促进了NS-1的糖消耗速率,提高了NS-1生物量和培养液pH值,明显促进了NS-1胞外雷公藤甲素的积累,最高含量是对照的4.2倍;NS-8菌丝体提取物诱导子对培养液pH值的影响不显著,NS-1糖消耗速率略有下降,对NS-1生长有一定的抑制作用,胞外雷公藤甲素含量低于对照.两种诱导子对NS-1胞内雷公藤甲素合成均未呈现明显的促进作用.因此,NS-8主要通过其代谢产物强化NS-1雷公藤甲素的合成.
In order to understand the triptolide synthesis ability of the endophytic fungi from Tripterygium wilfordii and the influence of the interaction between endophytic fungi on the triptolide synthesis,a triptolide-producing endophytic fungus NS-1 was screened from T.wilfordii,and was identified as Fusarium nisikadoi.By co-cultivating NS-1 with other endophytic fungi,the endophytic fungus NS-8 of the genus Penicilliumn was observed to significantly promote the triptolide synthesis of NS-1(P<0.05),which was 1.6 times than that of the control.Based on this,the effect of elicitors of endophytic fungus NS-8 on the triptolide synthesis of NS-1 was further studied by preparing and adding the elicitors of mycelium extract and fermentation broth.The results showed that NS-8 fermentation broth elicitor increased the sugar consumption rate,the biomass of NS-1 and the pH value of the culture broth.It significantly promoted the accumulation of NS-1 extracellular triptolide,with the highest content reaching 4.2 times of the control.The elicitors of NS-8 mycelium had little effect on the pH value of the culture medium,slightly decreased the sugar consumption rate of NS-1,and inhibited the growth of NS-1.The content of extracellular triptolide was lower than that of the control under the addition of NS-8 mycelium elicitors.The 2 types of elicitors did not significantly promote the intracellular triptolide synthesis of NS-1.Therefore,NS-8 enhanced the triptolide synthesis of NS-1 mainly through its metabolites.
作者
李国庆
宋萍
洪伟
吴承祯
封磊
LI Guoqing;SONG Ping;HONG Wei;WU Chengzhen;FENG Lei(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management,Fuzhou,Fujian 350002,China;Wuyi University,Wuyishan,Fujian 354300,China;College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2022年第1期104-111,共8页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省高校产学合作科技重大项目(2019N5006)
福建农林大学科技创新专项基金(CXZX2018105,CXZX2018133,CXZX2019078G).
