摘要
镰刀菌是太子参根腐病的致病菌之一,为了阐释太子参块根中可能分布的镰刀菌种类及其污染的毒素类型。该文对太子参块根中共分离的89株真菌,经ITS2序列鉴定,其中29株为镰刀属菌,占比达到32.5%。进而经β-Tubulin和EF-1α基因鉴定为镰刀菌属的Fusarium avenaceum、F.tricinctum、F.fujikuroi、F.oxysporum、F.graminearum 5个物种。采用LC-MS/MS检测技术对分离菌株产毒分析,发现产生ZEN、DON、T2的菌株分别有18、1、5株,占比分别为62.1%、3.4%、17.2%。其中菌株JK3-3能同时产生ZEN、DON、T23种毒素,菌株BH1-4-1、BH6-5、BH16-2能产生ZEN、T22种毒素。利用PCR检测技术发现,菌株JK3-3能检测到合成这3种毒素的Tri1、Tri7、Tri8、Tri13、PKS13、PKS146个关键合成酶基因,而菌株BH1-4-1、BH6-5、BH16-2能检测到合成ZEN、T22种毒素的Tri8、Tri13、PKS13、PKS144个关键合成酶基因。该结果表明毒素合成关键基因的检出与菌株产生毒素种类具有很高的相关性,毒素的合成严格受到菌株的遗传信息控制。该研究为太子参外源真菌毒素的针对性防控提供了数据基础以及开发PCR快速检测毒素污染的方法提供了可能。
Fusarium is the major pathogen of root rot of Pseudostellaria heterophylla.This study aims to explain the possible distribution of Fusarium species and the contamination of its toxin-chemotypes in tuberous root of P.heterophylla.A total of 89 strains of fungi were isolated from the tuberous root of P.heterophylla.Among them,29 strains were identified as Fusarium by ITS2 sequence,accounting for 32.5%.They were identified as five species of F.avenaceum,F.tricinctum,F.fujikuroi,F.oxysporum,and F.graminearum based onβ-Tubulin and EF-1αgenes.LC-MS/MS detected 18,1,and 5 strains able to produce ZEN,DON,and T2,which accounted for 62.1%,3.4%,and 17.2%,respectively.Strain JK3-3 can produce ZEN,DON,and T2,while strains BH1-4-1,BH6-5,and BH16-2 can produce ZEN and T2.PCR detected six key synthase genes of Tri1,Tri7,Tri8,Tri13,PKS14,and PKS13 in strain JK3-3,which synthesized three toxins of ZEN,DON,and T2.Four key synthase genes of Tri8,Tri13,PKS14,and PKS13 were detected in strains BH1-4-1,BH6-5,and BH16-2,which were responsible for the synthesis of ZEN and T2.The results showed that the key genes of toxin biosynthesis were highly correlated with the toxins produced by Fusarium,and the biosynthesis of toxin was strictly controlled by the genetic information of the strain.This study provides a data basis for the targeted prevention and control of exogenous mycotoxins in P.heterophylla and a possibility for the development of PCR for rapid detection of toxin contamination.
作者
袁青松
邓桃
安久春
郭兰萍
周世华
杨昌贵
韩邦兴
康传志
刘兴鹏
周涛
YUAN Qing-song;DENG Tao;AN Jiu-chun;GUO Lan-ping;ZHOU Shi-hua;YANG Chang-gui;HAN Bang-xing;KANG Chuan-zhi;LIU Xing-peng;ZHOU Tao(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;West Anhui University,Lu'an 237012,China;Guizhou Institute for Food and Drug Control,Guiyang 550004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第22期5792-5796,共5页
China Journal of Chinese Materia Medica
基金
中央本级重大增减支项目(2060302)
现代农业产业技术体系建设专项(CARS-21)
贵州省高层次创新型人才项目(黔科合平台人才[2018]5638-2)
贵州省科技创新人才团队项目(黔科合平台人才[2019]5611)
贵州省科技计划项目(黔科合支撑[2021]一般418)
贵州省科技成果应用及产业化项目(黔科合成果[2021]一般136)。
关键词
太子参
镰刀菌
毒素
毒素化学型
Pseudostellaria heterophylla
Fusarium
toxins
toxin chemotype
