摘要
目的建立葛根超高效液相色谱指纹图谱分析方法。方法色谱柱为Agilent ZORBAX Eclipse Plus C18(2.1 mm×100 mm,1.8µm);流动相为乙腈(A)-0.1%甲酸(B),梯度洗脱,流速0.3 ml/min;检测波长250 nm;柱温30℃;进样量2µl,采用中药指纹图谱相似度评价系统进行相似度评价。并使用SPSS软件进行聚类分析、主成分分析与偏最小二乘法分析,判断不同产地葛根成分差异。结果以葛根素为参照峰,标定了22个共有峰,指认了其中6个峰分别为3'-羟基葛根素、葛根素、3'-甲氧基葛根素、葛根素芹菜糖苷、大豆苷、大豆苷元色谱峰。25批样品中,24批样品相似度均在0.990以上,表明样品一致性良好。经聚类分析、主成分分析与偏最小二乘法分析,25批葛根药材可聚集为4~5类,并发现了3'-羟基葛根素等差异成分。基于层次分析法和多指标正交试验对葛根药材的提取工艺进行了优化,最佳提取工艺为:加入50%乙醇40 ml回流提取40 min。结论超高效液相色谱指纹图谱适用于葛根药材的质量评价。
Objective To establish the UPLC fingerprint analysis method for Puerariae Lobamle Radix.Methods The column was Agilent ZORBAX Eclipse Plus C18 column(2.1 mm×100 mm,1.8µm).The mobile phase consisted of acetonitrile(A)-0.1%formic acid(b),gradient elution,flow rate was 0.3 ml/min.The detection wavelength was 250 nm.The column temperature was set at 30℃.The sample volume was 2μl,and the similarity evaluation system of traditional Chinese medicine fingerprint was adopted.The cluster analysis,principal component analysis and partial least square method in SPSS software were used to judge the differences of Pueraria lobata from different habitats.Results With puerarin as reference peak,22 common peaks were calibrated,and 6 peaks were identified.The similarity of 25 batches of samples was above 0.990 except S22 was 0.935,which indicated that the samples were with good consistency.Through cluster analysis,principal component analysis and partial least square analysis,25 batches of Puerariae Lobamle Radix can be clustered into 4-5 categories,and different components such as 3'-hydroxy puerarin were found.The extraction process of Puerariae Lobamle Radix was optimized based on analytic hierarchy process and multi-index orthogonal test.The results showed that adding 50%ethanol 40 ml and refluxing for 40 min was the best extraction process.Conclusion UPLC fingerprint is suitable for Puerariae Lobamle Radix,and the results are reliable.It can be used as a quality evaluation method for Puerariae Lobamle Radix.
作者
赵开军
刁和芳
王海丽
Zhao Kaijun;Diao Hefang;Wang Haili(Zhongshan Pharmaceutical Co.,Ltd of Nanjing,Nanjing 210042,China;Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co.,LTD.,Nanjing 210042,China;The Classical Prescriptions Engineering Research Center of JiangSu,Nanjing 210042,China)
出处
《国际中医中药杂志》
2021年第12期1219-1225,共7页
International Journal of Traditional Chinese Medicine
基金
江苏省知识产权战略推进计划项目(ZT20210180-33)。
关键词
葛根
指纹图谱
葛根素
正交试验
层次分析法
Puerariae Lobamle Radix
Fingerprint
Puerarin
Orthogonal test
Analytic hierarchy process
