摘要
旨在建立并优化生菜子多糖的提取工艺,并对生菜子多糖进行体外降血脂活性的研究。以生菜子为试材,水醇法提取多糖,BP(反向传播)神经网络法与正交试验相结合建立生菜子多糖的提取工艺,并以体外结合胆酸盐实验研究生菜子多糖体外降血脂能力。最终得到生菜子多糖提取工艺条件为89℃,溶剂倍量44,提取1.6 h,提取4次,体外结合胆酸盐实验表明生菜子多糖及其不同醇沉片段均对牛磺胆酸钠(STC)、甘氨胆酸钠(SGC)、胆酸钠(SC)有较高结合率。最终发现BP神经网络法与正交试验相结合,有效减少试验次数,分析生菜子多糖提取因素变化规律并寻找最佳参数。生菜子多糖降血脂机制可能为肠道内结合胆酸盐,影响脂类物质吸收。
The aim is to establish and optimize the extraction process of polysaccharides from Lactuca Satya L.and to explore the in vitro hypolipidemic activity of the polysaccharides. Lactuca Satya L. was used as material,polysaccharides was extracted by water/ethanol method. The polysaccharide extraction process of Lactuca Satya L. was established by back propagation(BP) neural network and orthogonal design. The in vitro hypolipidemie of polysaccharide in Lactuca Satya L. was studied by in vitro binding of cholates. The optimal condition for Lactuca Satya L. polysaccharide extraction was at 89℃ with solvent amount of 44 times in 1.6 h and 4 extracting times. The polysaccharides in Lactuca Satya L. and their alcohol-precipitated fractions had better cholate binding capacity with sodium taurocholate(STC), glychocholic acid sodium salts(SGC), sodium cholate(SC). BP neural network combined with orthogonal test could reduce experiment times effectively,analyze the change rules of Lactuca Satya L. polysaccharide extraction factors, and obtain the optimal extraction parameters. The hypolipidemic mechanism of Lactuca Satya L. polysaccharide might be the combination of cholic acids in intestine, which affected the lipids absorption.
作者
杨丰榕
高建萍
李春燕
Yang Fengrong;Gao Jianping;Li Chunyan(College of Pharmacy,Inner Mongolia Medical University,Hohhot 010110)
出处
《农学学报》
2021年第11期81-87,共7页
Journal of Agriculture
基金
内蒙古医科大学实验室开放项目“基于BP神经网络的黑苣胜化学成分提取方法研究及其药理活性初探”(2018ZN35)。
关键词
生菜子
多糖
提取工艺
正交试验
BP神经网络
降血脂
Lactuca Satya L.
Polysaccharide
extraction process
Orthogonal Design
BP Neural Network
Hypolipidemic
