摘要
目的:建立恒山黄芪的氢核磁共振指纹图谱,为恒山黄芪的质量控制提供依据。方法:采用超声波复合酶法提取恒山黄芪化学成分;以氘代氯仿为提取溶剂,在25℃下采用标准的预饱和脉冲序列压制水峰建立10批不同来源恒山黄芪的氢核磁共振指纹图谱,通过"加标准品定性"实验、数据库比对和相关文献比对进行信号归属,采用相关系数法计算10批不同来源恒山黄芪;H-NMR指纹图谱的相似度,并进行重复性、精密度及稳定性考察,基于SIMCA软件进行主成分分析,进行药材的质量评价。结果:从恒山黄芪药材中检测分析出24种化合物,包括三萜皂苷、异黄酮、氨基酸、有机酸、糖等。10批恒山黄芪的指纹图谱相似度均>0.9,平均为0.975,与蒙古黄芪和甘肃黄芪对比分析,恒山黄芪的异黄酮含量高于两者。PCA结果显示,前三个主成分的累积贡献率为89.8%,与相似度评价结果基本一致。结论:建立的;H-NMR方法具有快速简便的优点,可为恒山黄芪的质量控制及使用提供参考。
Objective: To establish a hydrogen nuclear magnetic resonance(;H-NMR) fingerprint of Hengshan Astragalus membranaceus, and provide a basis for its quality control. Method: The chemical components were extracted by ultrasonic assisted enzymatic method. Using deuterated chloroform as the extraction solvent, a standard pre-saturated pulse sequence was used to suppress water peaks at 25 ℃ to establish;H-NMR fingerprints of 10 batches of A. membranaceus from different sources. The signal attribution was assigned by the qualitative standard addition test, database comparison and related literature comparison. The correlation coefficient method was used to calculate the similarity of;H-NMR fingerprints, and the repeatability, precision and stability were investigated. Principal component analysis based on SIMCA was used to evaluate the quality of medicinal materials. Results: 24 compounds were detected and analyzed from Hengshan A. membranaceus, including triterpene saponins, isoflavones, amino acids, organic acids, sugars and so on. The similarities of fingerprints were higher than 0.9 with an average of 0.975. Compared with Mongolian Astragalus membranaceus and Gansu Astragalus membranaceus, the content of isoflavones in Hengshan Astragalus membranaceus was higher than both.PCA results showed that the cumulative contribution rate of the first three principal components was 89.8%, which was basically consistent with the similarity evaluation results. Conclusion: The established;H-NMR method had the advantages of quickness and simplicity, which could provide reference for the quality control of Hengshan A. membranaceus.
作者
张弘弛
刘瑞
巴德方
李慧
杨阳
周凤
ZHANG Hongchi;LIU Rui;BA Defang;LI Hui;YANG Yang;ZHOU Feng(School of Agriculture and Life Sciences,Shanxi Datong University,Applied Biotechnology Institute,Shanxi Datong University,Datong 037009,China)
出处
《食品工业科技》
CAS
北大核心
2022年第1期47-55,共9页
Science and Technology of Food Industry
基金
山西省应用基础研究计划项目(编号:201901D111313)
山西省高等学校科技创新项目(编号:2020L0469)
山西大同大学科研基金项目(编号:2019K15)。
关键词
恒山黄芪
氢核磁共振
异黄酮
皂苷
指纹图谱
主成分分析
Hengshan Astragalus membranaceus
hydrogen nuclear magnetic resonance
isoflavones
saponins
fingerprint
principal component analysis
