摘要
水体中残留的H_(2)O_(2)进入人体后会转化为含氧自由基,进而对机体造成氧化损伤并引发病变,因此实现水体中的H_(2)O_(2)检测具有重要意义.制备了具有较高类过氧化物酶活性的MoS_(2)-Fe_(3)O_(4)复合物水凝胶,并构建比色传感平台实现了水体中H_(2)O_(2)的灵敏检测.在H_(2)O_(2)-TMB显色体系中,Fe_(3)O_(4)与MoS_(2)纳米片复合协同催化H_(2)O_(2)产生含氧自由基,水凝胶三维结构可有效抑制MoS_(2)-Fe_(3)O_(4)堆叠,促进活性位点的暴露,进一步提高对H_(2)O_(2)的催化能力.动力学常数显示,MoS_(2)-Fe_(3)O_(4)复合物水凝胶催化H_(2)O_(2)底物的最大速度是辣根过氧化物酶(HRP)的1.5倍.基于较高的H_(2)O_(2)催化活性,水体中H_(2)O_(2)检测限达到0.28μmol/L,检测线性范围为3.0~200μmol/L,并采用加标回收法对自来水、湖水和海水等实际水体中的H_(2)O_(2)进行了检测.
H_(2)O_(2)residual in water will be converted into oxygen-containing free radicals after entering body,which will cause oxidative damage to body and pathological changes.Therefore,it is of great significance for H_(2)O_(2)detection in water.MoS_(2)-Fe_(3)O_(4)composite hydrogel with remarkably enhanced peroxidase-like catalytic activity is synthesized,and the sensitive detection of H_(2)O_(2)in water is realized through the construction of colorimetric sensors platform.In the H_(2)O_(2)-TMB color system,the generation of oxygen-containing free radicals by H_(2)O_(2)is catalyzed by MoS_(2) and Fe_(3)O_(4) nanosheets synergistically.Meanwhile,the construction of 3D structure of hydrogel can inhibit the stacking of MoS_(2)-Fe_(3)O_(4),which is benefit for the exposure of active sites,and highly enhance catalytic activity for H_(2)O_(2).The kinetic constants show that MoS_(2)-Fe_(3)O_(4)composite hydrogel has maximum catalytic velocity for substrates of H_(2)O_(2),which is about 1.5 times higher than that of HRP(horseradish peroxidase).Based on high catalytic activity for H_(2)O_(2),detection is conducted with a linear relationship between absorbance and H_(2)O_(2)concentrations,ranging from 3.0 to 200μmol/L,and the limit of detection(LOD)is 0.28μmol/L.H_(2)O_(2)in running water,lake,sea is detected by marking recovery method.
作者
崔梦雨
党雪明
赵慧敏
CUI Mengyu;DANG Xueming;ZHAO Huimin(Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China)
出处
《大连理工大学学报》
CAS
CSCD
北大核心
2022年第1期28-36,共9页
Journal of Dalian University of Technology
基金
国家自然科学基金资助项目(2177070493).
