摘要
目的:通过麻芥巴布膏干预哮喘小鼠,观察其肺、皮、肠组织中上皮源性细胞因子的变化。方法:将C57/BL6雌性小鼠分为空白对照组、哮喘模型组、地塞米松组、麻芥巴布膏组。使用卵白蛋白(ovalbumin,OVA)为致敏剂诱导建立哮喘小鼠模型,计算脾指数,使用苏木精-伊红染色观察肺组织的病理改变,酶联免疫吸附测定检测血清中免疫球蛋白E(immunoglobulin E,IgE),及肺、皮、肠组织中胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)的含量;实时荧光定量PCR法检测皮、肠组织中TSLP mRNA、白细胞介素-33(IL-33)mRNA的表达。结果:与空白对照组比较,哮喘模型组小鼠脾指数增高,并出现血管内充血水肿,炎性细胞浸润和支气管壁增厚的病理变化,血清中IgE的含量增多,TSLP含量在肺、皮组织中增多,肠组织中没有明显变化,TSLP mRNA的表达在皮、肠组织中显著上调,而IL-33 mRNA的表达在皮组织中显著上调(P<0.05),但在肠组织中无显著性差异(P>0.05)。与模型对照组比较,麻芥巴布膏可降低脾指数和血清中IgE的含量,并可改善肺组织病理损伤,减少肺、皮、肠组织中TSLP的含量,增加皮组织中TSLP mRNA表达,下调皮组织中IL-33 mRNA表达以及肠组织中TSLP mRNA、IL-33 mRNA的表达,差异均有统计学意义(P<0.05)。在肺组织与皮组织间,实验各组TSLP含量的相关性为0.689,肺组织与肠组织间的相关性为−0.163,皮组织和肠组织间的相关性为0.422,差异均无统计学意义(P>0.05)。结论:麻芥巴布膏可以降低OVA诱导的哮喘小鼠“肺-皮-肠”轴上皮源性细胞因子的含量,从而改善气道炎症,达到治疗哮喘的作用。
Objective:To evaluate the effect of Majie Pingchuan(MJPC)cataplasm on the levels of epithelial-derived cytokines in the lung,skin and intestine of asthmatic mice.Methods:C57/BL6 female mice were randomly divided into four groups:the control group,the asthma model group,the dexamethasone group and the MJPC cataplasm group.Ovalbumin(OVA)was used for the establishment of asthmatic mouse models.The spleen index was calculated,and hematoxylineosin staining was used to observe the pathological change in lung tissues.The IgE level in the mouse serum and the content of thymic stromal lymphopoietin(TSLP)in the lung,skin and intestine were detected by ELISA.The expressions of TSLP mRNA and IL-33 mRNA in the skin and intestinal tissue were detected by qRT-PCR.Results:Compared with the control group,the spleen index of mice in the asthma model group was increased.In addition,vascular congestion and edema,inflammatory cell infiltration and bronchial wall thickening were observed.The IgE level in the serum was significantly elevated,and the content of TSLP in lung and skin tissues was increased without changes in intestinal tissues.The expression of TSLP mRNA was upregulated in skin and intestinal tissues while the expression of IL-33 mRNA upregulated in skin tissues(P<0.05)without significant changes in intestinal tissues.Compared with the model group,MJPC cataplasm could decrease the spleen index and the IgE level in the mouse serum,improve the pathological damages of lung tissues in asthmatic mice,reduce the content of TSLP in lung,skin and intestinal tissues,increase the expression of TSLP mRNA in skin tissues,and downregulate the expression of IL-33 mRNA in skin tissues and the expres-sions of TSLP mRNA and IL-33 mRNA expression in intestinal tissues(P<0.05).Pearson correlation coefficient of the content of TSLP between the lung and the skin was 0.689,between the lung and the intestine was−0.163,and between the skin and the intestine was 0.422,and the differences were not statistically significant(P>0.05).Conclusion:MJPC cataplasm can improve airway inflammation by inhibiting the content of epithelial-derived cytokines on the lung-skin-intestine axis of asthmatic mice,and achieve the effect of treating asthma.
作者
石涵芬
冯天意
张双
纪雯婷
王庆国
王雪茜
SHI Han-fen;FENG Tian-yi;ZHANG Shuang;JI Wen-ting;WANG Qing-guo;WANG Xue-qian(Beijing University of Chinese Medicine,Beijing 100105,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)
出处
《海南医学院学报》
CAS
2022年第1期13-18,共6页
Journal of Hainan Medical University
基金
国家自然科学基金资助项目(No.81874448)。
