摘要
目的:对美味牛肝菌色素进行大孔树脂纯化并研究其抗氧化性。方法:通过静态和动态试验考察了树脂类型、上样浓度、pH、上样流速、乙醇体积分数及洗脱流速对美味牛肝菌色素吸附—解吸性能的影响,确定最佳纯化工艺条件;并采用红外光谱及DPPH·、ABTS^(+)·及·OH清除能力研究纯化后色素的特征结构和抗氧化性。结果:AB-8大孔树脂纯化美味牛肝菌色素效果最好,最佳纯化工艺条件为:样液质量浓度1.5 mg/mL、pH 2.0、上样流速3.0 mL/min、乙醇体积分数70%、解吸流速2.0 mL/min,该条件下,美味牛肝菌色素的纯化效率是269%。纯化后美味牛肝菌色素清除DPPH·、ABTS^(+)·及·OH的IC_(50)值分别达到(0.081±0.001),(0.017±0.011),(0.119±0.001)mg/mL,其中清除·OH能力超过维生素C。结论:AB-8大孔树脂适用于美味牛肝菌色素的分离纯化,纯化后色素具有良好的抗氧化活性。
Objective:The aim of this work is to obtain the optimal purification process of pigment from Boletus edulis by macroporous resin,and study its antioxidant activity.Methods:Based on the static and dynamic test,the influence of resin type,sample concentration,pH,loading flow ratio,ethanol concentration and elution flow ratio on the adsorption and desorption activity of Boletus edulis pigment were investigated to determine the optimal purification conditions.Then the structure,the DPPH·,ABTS^(+)·and·OH scavenging activity of the Boletus edulis pigment purified was also evaluated.Results:AB-8 was the most suitable type of macroporous resin and the optimum purification conditions were as follows:the pigment concentration of 1.5 mg/mL,pH of 2.0,the sample flow ratio of 2.0 mg/mL,ethanol concentration of 70%(V/V)and the elution flow ratio of 2.0 mL/min.Under these conditions,the purification efficiency of Boletus edulis pigment was 269%.The IC_(50) of the final purified product for scavenging DPPH·,ABTS^(+)·and·OH were(0.081±0.001),(0.017±0.011)and(0.119±0.001)mg/mL respectively.Especially,the·OH scavenging ability was higher than V_(C).Conclusion:AB-8 resin was suitable for separation and purification of Boletus edulis pigment,and the pigment after purification revealed strong antioxidant activity.
作者
郭磊
资璐熙
崔琦
周宇
邱蕙婷
阚欢
GUO Lei;ZI Lu-xi;CUI Qi;ZHOU Yu;QIU Hui-ting;KAN Huan(School of Life Science,Southwest Forestry University,Kunming,Yunnam 650224,China)
出处
《食品与机械》
北大核心
2022年第1期152-158,163,共8页
Food and Machinery
基金
云南省科技厅基础研究计划项目(编号:202101AT070042)
云南省科技计划项目(编号:2018ZG004)
国家级大学生创新训练项目(编号:202010677107)。
关键词
美味牛肝菌
色素
大孔树脂
纯化
结构
抗氧化
Boletus edulis
pigment
macroporous resin
purification
structure
antioxidant
