摘要
建立了高效液相色谱-示差折光检测法(High Performance Liquid Chromatography Differential Refractive Detector,HPLC-RID)测定蜂蜜中果糖、葡萄糖、蔗糖、麦芽糖及乳糖5种还原糖含量的分析方法。蜂蜜试样用水提取,摇匀过滤后,经Kromasil 100-5-NH_(2)色谱柱分离,用示差折光检测器测定。结果表明,果糖、葡萄糖、蔗糖、麦芽糖及乳糖在1.0~10.0 mg/mL线性关系良好,5种还原糖的方法检出限均为0.5 g/100 g,加标回收率为82.4%~109.7%,相对标准偏差RSD为0.87%~3.77%。该方法前处理过程简单、准确度高、精密度好,适用于蜂蜜中5种还原糖的定量检测。
The method was established for the determination of fructose,glucose,sucrose,maltose and lactose in honey by HPLC-RID.Honey samples were extracted with water,shaken and filtered,separated by Kromasil 100-5-NH_(2) chromatographic column and determined by differential refractive detector.The results showed that the linear relationship among fructose,glucose,sucrose,maltose and lactose was good in the concentration range of 1.0~10.0 mg/mL.The detection limits of the five reducing sugars were 0.5 g/100 g,the spiked recoveries were 82.4%~109.7%,and the relative standard deviation RSD was 0.87%~3.77%.The method has the advantages of simple pretreatment process,high accuracy and good precision.It is suitable for the quantitative determination of the above five reducing sugars in honey.
作者
赵芳
ZHAO Fang(Jinzhong General Inspection and Testing Centre,Jinzhong 030600,China)
出处
《食品安全导刊》
2021年第33期92-94,共3页
China Food Safety Magazine
关键词
蜂蜜
还原糖
高效液相色谱-示差折光检测器法
honey
reducing sugar
high performance liquid chromatography differential refractive detector
