摘要
目的研究IgA肾病(IgAN)患者外周血microRNA-223(miR-223)、NOD样受体蛋白3(NLRP3)水平与肾间质纤维化(RIF)的相关性。方法选取2018年1月—2020年1月在重庆市綦江区人民医院经肾组织病理检查确诊为原发性IgAN患者164例作为研究对象,按照RIF程度不同分为3组:RIF T0组、RIF T1组、RIF T2组。另取同期该院健康体检志愿者95例作为对照A组,以及非IgAN患者80例作为对照B组。比较各组受试者一般资料。采用实时荧光定量聚合酶链反应测定受试者外周血miR-223、NLRP3 mRNA相对表达量;酶联免疫吸附试验(ELISA)测定受试者外周血RIF指标[转化生长因子β_(1)(TGF-β_(1))、单核细胞趋化蛋白1(MCP-1)、转化生长因子α(TGF-α)、白细胞介素6(IL-6)、缺氧诱导因子α(HIF-α)];Spearman法分析miR-223、NLRP3 mRNA与RIF指标的相关性。结果与对照A组相比,对照B组、RIF T0组、RIF T1组、RIF T2组患者miR-223相对表达量依次降低(P<0.05),而NLRP3 mRNA、TGF-β_(1)、MCP-1、TGF-α、IL-6、HIF-α依次升高(P<0.05)。Spearman相关性分析结果显示,IgAN患者TGF-β_(1)、MCP-1、TGF-α、IL-6、HIF-α与miR-223呈负相关(P<0.05),而与NLRP3 mRNA呈正相关(P<0.05)。结论IgAN患者外周血miR-223低表达,与RIF指标呈负相关;NLRP3 mRNA高表达,与RIF指标呈正相关,提示miR-223、NLRP3可能参与IgAN患者RIF进程。
Objective To study the correlations between the levels of microRNA-223(miR-223)and NODlike receptor protein 3(NLRP3)and renal interstitial fibrosis(RIF)in patients with IgA nephropathy(IgAN).Methods From January 2018 to January 2020,164 patients with primary IgAN diagnosed via renal histopathology were selected.According to the degree of RIF,they were divided into RIF T0 group,RIF T1 group and RIF T2 group.In addition,95 healthy volunteers during the same period were selected as the control group A and 80 patients with non-IgA nephropathy as the control group B.The general data of the subjects were compared among the groups.The mRNA expression levels of miR-223 and NLRP3 were measured via quantitative real-time polymerase chain reaction(qRT-PCR),while the levels of RIF-related indexes[transforming growth factorβ_(1)(TGF-β_(1)),monocyte chemoattractant protein(MCP-1),transforming growth factorα(TGF-α),interleukin-6(IL-6),and hypoxia-inducible factorα(HIF-α)]were measured via enzyme-linked immunosorbent assay(ELISA).The Spearman method was used to analyze the correlations between the m RNA levels of miR-223 and NLRP3 and RIF-related indexes.Results Compared with control group A,the mRNA levels of miR-223 were lower in control group B,RIF T0 group,RIF T1 group and RIF T2 group and decreased successively in these groups(P<0.05),while the mRNA levels of NLRP3,TGF-β_(1),MCP-1,TGF-α,IL-6 and HIF-αwere higher and increased successively in these groups(P<0.05).Spearman analysis showed that the levels of TGF-β_(1),MCP-1,TGF-α,IL-6 and HIF-αwere negatively correlated with the mRNA level of miR-223,but were positively correlated with the mRNA level of NLRP3 in IgAN patients(all P<0.05).Conclusions The miR-223 is lowly expressed in the serum of patients with IgAN,and is negatively correlated with RIF-related indexes.On the contrary,the NLRP3 is highly expressed and positively correlated with RIF-related indexes.Thus,miR-223 and NLRP3 may be involved in the progression of RIF in IgAN patients.
作者
朱付英
李瑾
阎其均
周晓莉
Fu-ying Zhu;Jin Li;Qi-jun Yan;Xiao-li Zhou(Department of Clinical Laboratory,People's Hospital of Qijiang District,Chongqing 401420,China;Department of Cardiovascular Medicine,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)
出处
《中国现代医学杂志》
CAS
北大核心
2022年第2期32-37,共6页
China Journal of Modern Medicine
基金
重庆市2015年医学科研计划项目(No:2015-31)。
