摘要
目的为大叶千斤拔药材等级评价的修订提供科学依据。方法收集不同产地大叶千斤拔样品30批,对其直径、杂质、水分、总灰、浸出物、染料木苷含量进行测定;参照2020年版《中华人民共和国药典》第四部通则2201及采用高效液相色谱法分别测定浸出物、染料木苷含量;运用Excel和SPSS统计软件对数据进行相关性分析、聚类分析,结合生产和市场实际运用,为制订大叶千斤拔药材商品等级评价提供依据;建立大叶千斤拔抑菌生物评价方法,并通过聚类分析和PCA分析进行不同产地药材生物活性情况分析。结果通过相关性分析、聚类分析,筛选出直径、浸出物、染料木苷含量3个指标,并结合生产实际划分选货和统货。生物活性评价表明不同产区大叶千斤拔的抑菌效果存在差异。结论所建立的大叶千斤拔药材商品等级划分综合考虑了外观性状和成分含量,该评价方法适合于大叶千斤拔药材的等级判断,为后期基于功能性成分的含量和生物活性评价制订更加全面合理的大叶千斤拔药材商品等级标准提供依据。
Objective To provide scientific basis for institution of medicinal materials grade evaluation of Moghania macrophylla.Methods Thirty samples of Moghania macrophylla from different producing areas were collected. The diameter, impurity, moisture,total ash, extract and content of genistein were determined. Excel and SPSS statistical software were used to carry out correlation analysis and cluster analysis on the data, combined with the actual application of production and market, to provide a basis for formulating the commercial grade evaluation of Moghania macrophylla. A biological evaluation method for the antimicrobial activity of Moghania macrophylla was established, and cluster analysis and PCA analysis were carried out to analyze the biological activity of medicinal materials from different habitats. Results Through correlation analysis and cluster analysis, three indexes including diameter,extract and genistein content were screened out, and the selected goods and the unified goods were divided according to the actual production. The biological activity evaluation showed that there were differences in the bacteriostatic effects of Moghania macrophylla in different producing areas. Conclusion The established commercial grade classification of Moghania macrophylla takes comprehensive consideration of appearance and component content, and the evaluation method is suitable for grade judgment of Moghania macrophylla, and provides a basis for formulating a more comprehensive and reasonable commercial grade standard of Moghania macrophylla based on the content and biological activity evaluation of functional components.
作者
郑元青
张鹏
何娟娟
付卡利
张英帅
何风艳
牛明
龚云
ZHENG Yuanqing;ZHANG Peng;HE Juanjuan;FU Kali;ZHANG Yingshuai;HE Fengyan;NIU Ming;GONG Yun(Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou,Hunan 412000,China;National Institutes for Food and Drug Control,Beijing 100050,China;The Fifth Medical Center of General Hospital of PLA,Beijing 100071,China)
出处
《湖南中医药大学学报》
CAS
2022年第2期200-205,共6页
Journal of Hunan University of Chinese Medicine
基金
国家中药标准化项目(ZYBZH-C-HUN-21)。
关键词
千斤拔
等级评价
高效液相色谱法
染料木苷
生物活性评价
Moghania macrophylla
grade evaluation
high performance liquid chromatography
genistein
bioactivity evaluation
