摘要
目的:研究上调Notch胞内区域(Notch Intracellular Domain,NICD)对人牙周膜干细胞增殖及迁移能力的影响。方法:将细胞分为上调组、对照组及空白组,使用MTT比色法检测细胞增殖能力,流式细胞仪检测细胞凋亡能力,采用细胞划痕实验检测细胞迁移情况,并使用Western blot检测人牙周膜干细胞中Wnt/β-catenin信号通路蛋白(TGF-β、BMP-2、β-caten、LEF-1、Cyclin D1、Caspase-3、Bax、Bcl-2)表达量。结果:对照组各时间点的人牙周膜干细胞增殖率、碱性磷酸酶活性、迁移、黏附个数、TGF-β、BMP-2、β-caten、LEF-1、Cyclin D1、Bcl-2蛋白表达量均低于空白组,且凋亡率、Caspase-3、Bax蛋白表达量高于空白组,差异具有统计学意义(P<0.05)。上调组各时间点人牙周膜干细胞增殖率、碱性磷酸酶活性、迁移、黏附个数、TGF-β、BMP-2、β-caten、LEF-1、Cyclin D1、Bcl-2蛋白表达量均高于空白组和对照组,且凋亡率、Caspase-3、Bax蛋白表达量均低于空白组和对照组,差异具有统计学意义(P<0.05)。结论:上调NICD基因能促进人牙周膜干细胞的增殖和迁移,其机制可能与Wnt/β-catenin通路有关。
Objective To investigate the effect of up regulating Notch intracellular domain(NICD)on the proliferation and migration of human periodontal ligament stem cells.Methods NICD cells were divided into the up regulation group,the control group and the blank group.MTT colorimetry was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,cell migration was detected by cell scratch test,and the expression of Wnt/β-catenin signaling pathway protein in human periodontal ligament stem cells was detected by Western blot(TGF-β、BMP-2,β-caten,LEF-1,Cyclin D1,Caspase-3,Bax,Bcl-2).Results The proliferation rate,alkaline phosphatase activity,migration,number of adhesion and TGF-β,BMP-2,β-caten,LEF-1,Cyclin D1,Bcl-2 protein expression of human periodontal ligament stem cells in the control group at each time point were lower than those in the blank group,and the apoptosis rate,the expression of Caspase-3 and Bax protein in the blank group were higher than those in the blank group,the differences were statistically significant(P<0.05).The proliferation rate,alkaline phosphatase activity,migration,number of adhesion and TGF-β,BMP-2,β-caten,LEF-1,Cyclin D1,Bcl-2 protein expression of human periodontal ligament stem cells in the up regulation group at each time point were higher than those in the blank group and the control group(P<0.05).The apoptosis rate,the expression of Caspase-3 and Bax protein in the up regulation group were lower than those in the blank group and the control group(P<0.05).Conclusion Upregulation of NICD gene can promote the proliferation and migration of human periodontal ligament stem cells,and its mechanism may be related to Wnt/β-catenin pathway.
作者
张大灵
王源元
刘莉
周嫣
李华
ZHANG Daling;WANG Yuanyuan;LIU Li;ZHOU Yan;LI Hua(Department of Orthodontics,People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China;Department of Stomatology,Affiliated Stomatological Hospital of Guilin Medical University,Guilin 541000,Guangxi,China;Department of Oral and Maxillofacial Surgery,Hospital of Stomatology,Guangxi Medical University,Nanning 530021,Guangxi,China)
出处
《中国美容医学》
CAS
2022年第1期97-101,共5页
Chinese Journal of Aesthetic Medicine
基金
广西壮族自治区卫生和计划生育委员会自筹经费科研课题(编号:Z20180729)。
关键词
Notch胞内结构域
人牙周膜干细胞
增殖
凋亡
迁移
信号通路
Notch intracellular domain
human periodontal ligament stem cells
proliferation
apoptosis
migration
signaling pathways
