摘要
建立了一种近红外漫反射光谱快速测定不同产地雪菊中总黄酮含量的方法。以10个不同产地的100份雪菊样品为对象,采用NaNO_(2)-Al(NO_(3))_(3)比色法对来自不同产地的雪菊进行总黄酮含量测定;通过不同产地雪菊样品的近红外光谱图和对应的总黄酮含量,运用偏最小二乘法(PLS)建立了雪菊总黄酮含量的快速测定模型并对模型进行了验证。试验结果表明,采用连续小波变换与标准正态变换的方法进行光谱预处理,所建立的定量模型稳健有效,模型的相关系数为0.9862,交叉验证均方根误差为0.6259,预测残差值为4.0907,预测回收率为93.06%~104.64%。说明通过近红外漫反射光谱结合化学计量学方法能够实现快速测定不同产地雪菊中总黄酮的含量,该方法预测效果良好,结果准确,对快速、无损地测定其他植物样品中总黄酮的含量具有一定的借鉴意义。
A method for the rapid determination of total flavonoids in snow chrysanthemum from different places by near-infrared diffuse reflectance spectroscopy was established.Taking 100 samples of snow chrysanthemum from 10 different producing areas as the research object,NaNO_(2)-Al(NO_(3))_(3)colorimetric method is used to determine the total flavonoid content of snow chrysanthemum from different producing areas.Based on the near-infrared spectra and the corresponding total flavonoid content data of the snow chrysanthemum samples from different origins,a rapid determination model of snow chrysanthemum total flavonoids by using partial least squares(PLS)was established and verified.The correlation coefficient R of the established model is 0.9862,the cross validation of root mean square error RMSECV is 0.6259,the predicted residual value is 4.0907,and the predicted recovery rate is 93.06%~104.64%.Therefore,the method of near-infrared diffuse reflectance spectroscopy combined with chemometrics can quickly determine the content of total flavonoids in snow chrysanthemum of different origins.This method has the advantages of good prediction effect and accurate results,and it has certain reference significance for the rapid and non-destructive determination of total flavonoids in other plant samples.
作者
冯昱龙
彭娟
贺小刚
于佳萍
韩想
楚刚辉
FENG Yu-long;PENG Juan;HE Xiao-gang;YU Jia-ping;HAN Xiang;CHU Gang-hui(Xinjiang Special Medicine and Edible Plant Resources Chemistry Laboratory,School of Chemistry and Environmental Science,Kashgar University,Kashgar 844000,China)
出处
《化学试剂》
CAS
北大核心
2022年第2期279-284,共6页
Chemical Reagents
基金
国家自然科学基金资助项目(21765012)
新疆维吾尔自治区高校科研计划自然科学重点资助项目(XJEDU2020I016)。
关键词
雪菊
近红外光谱
总黄酮
快速测定
化学计量学
无损
snow chrysanthemum
near infrared spectroscopy
total flavonoids
rapid determination
stoichiometry
non-destructive
