摘要
目的探究罗米司亭对血小板减少性紫癜(ITP)小鼠骨髓单个核细胞(BMNC)中Notch信号通路,以及外周血单个核细胞(PBMC)中转录因子结合蛋白-3(GATA-3)和程序性细胞死亡受体-1(PD-1)水平的影响。方法48只小鼠随机均分为对照组、ITP组和罗米司亭组。腹腔注射抗血小板抗体建立ITP模型,罗米司亭组皮下注射罗米司亭2μg/kg。分别检测3组小鼠的血小板计数变化情况。使用实时荧光定量聚合酶链反应(qRT-PCR)、Western blot、流式细胞术检测BMNC中Notch和Jagged1,以及PBMC中GATA-3和PD-1水平。结果建模后小鼠血小板计数显著降低(P<0.05),罗米司亭干预第7天和第14天罗米司亭组血小板计数显著高于ITP组(P<0.05)。建模后小鼠BMNC中Notch信号通路表达水平显著升高(P<0.05),罗米司亭组Notch、Jagged1蛋白和mRNA表达水平显著低于ITP组(P<0.05)。建模后小鼠GATA-3 mRNA和PD-1水平显著降低(P<0.05),罗米司亭组GATA-3 mRAN和PD-1水平显著高于ITP组(P<0.05)。结论罗米司亭可提高ITP小鼠血小板计数,并且抑制BMNC中Notch信号通路表达水平,上调PBMC中GATA-3和PD-1表达。
Aim To explore the effects of Romipristine on Notch signal in mononuclear cells(BMNC)in mice with thrombocytopenic purpura,transfer factor binding protein-3(GATA-3)in bone marrow monouclear cells(PBMC)and serumprogrammed cell death receptor-1(PD-1)levels.Methods Forty-eight mice were randomly divided into 3 groups,16 each,namely the control group,the model group and the study group.The immune thrombocytopenic purpura was established by intraperitoneal injection of anti-platelet antibody,and the study group was injected with 2μg/kg of rilastast.The changes in platelet counts of the three groups of mice were examined separately.Notch signaling,GTA-3 of PBMC and serum PD-1 levels in BMNC were detected by real time fluorescence quantitative polymerase chain reaction(qRT-PCR),Western blot or flow cytometry.Results The platelet count of mice was significantly decreased after modeling(P<0.05).The platelet count of the study group was significantly higher than that of the model group at 7 days after intervention and 14 days after intervention(P<0.05).The expression level of Notch signaling pathway in mouse BMNC was significantly up-regulated after modeling(P<0.05).The expression levels of Notch,Jagged1 protein and mRNA in the study group were significantly lower than those in the model group(P<0.05).The levels of GATA-3 and PD-1 in mice were significantly decreased after modeling(P<0.05).The GATA-3 mRAN and PD-1 levels of the study group were significantly higher than the model group(P<0.05).Conclusion Romipristine can increase the platelet count of immune thrombocytopenic purpura mice,and inhibit the expression of Notch pathway in BMNC,and up-regulate the expression of GATA-3 and serum PD-1 in PBMC.
作者
张扬
王文斌
ZHANG Yang;WANG Wenbin(Department of Oncology and Hematology,Bishan District Peoples Hospital,Chongqing 402760,China;Haifu Tumor Center,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)
出处
《中南医学科学杂志》
CAS
2022年第1期59-62,共4页
Medical Science Journal of Central South China
基金
重庆市科卫联合医学科研项目(2018QNXM042)。
