摘要
为研究不同浓度的纳米级二氧化钛对圆形盘肠溞(Chydorussphaericus)急性毒性的影响,以纳米级二氧化钛为材料,通过液体培养试验,观察圆形盘肠溞的生存和死亡情况,并测定抗氧化酶、丙二醛(malondialdehyde,MDA)等的指标。结果表明,纳米二氧化钛对圆形盘肠溞的急性毒性为2 mg/L(24 h EC50);之后,随着纳米级二氧化钛浓度越高,圆形盘肠溞死亡率越高。另外,在24 h时,经1 mg/L的纳米二氧化钛处理后,圆形盘肠溞的超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase,POD)活性最高,分别比0 mg/L的纳米二氧化钛处理提高了133.82%和23.38%,而圆形盘肠溞的MDA呈现出逐渐上升的趋势;在12 h、48 h时,随着纳米二氧化钛浓度的升高,SOD和POD活性呈现出下降的趋势,而圆形盘肠溞的MDA含量在12 h时,呈现“先升后降”的趋势。因此,在24 h时,纳米二氧化钛处理对圆形盘肠溞的生长有显著的抑制作用,同时圆形盘肠溞的机体自我修复能力较强。而随着时间的增加,超过圆形盘肠溞自我修复的范围,机体损伤严重。
In order to study the effects of different concentrations of nano-TiO_(2) on the acute toxicity of Chydorussphaericus,nanometer oxide was used in this experiment.The survival and death of Chydorussphaericus were observed by liquid culture test with nano-TiO_(2),and the indexes of antioxidant enzymes and malondialdehyde(MDA)were determined.The results showed that the acute toxicity of nano-TiO_(2) to Chydorussphaericus was 2 mg/L in 24 h.After that,the higher the concentration of nano-TiO_(2),the higher the mortality of Chydorussphaericus.In addition,at 24 h,the activities of superoxide dismutase(SOD)and peroxidase(POD)of Chydorussphaericus treated with 1 mg/L nano-TiO_(2) were the highest,which were 133.82%and 23.3%higher than those treated with 0 mg/L nano-TiO_(2),respectively,while the MDA of Chydorussphaericus showed an increasing trend.At 12 h and 48 h,with the increase of nano-TiO_(2) concentration,the activities of SOD and POD showed a downward trend,while the MDA content of Chydorussphaericus showed a trend of"first increase and then decrease"at 12 h.Therefore,the growth of Chydorussphaericus was significantly inhibited by nano-TiO_(2) treatment at 24 h,and the self-healing ability of Chydorussphaericus was strong.However,with the increase of time,the self-repair range of Chydorussphaericus was exceeded,resulting in serious body damage.
作者
孔昊
陈杰
王佳琪
张琦
刘嘉慧
张亮
KONG Hao;CHEN Jie;WANG Jia-qi;ZHANG Qi;LIU Jia-hui;ZHANG Liang(School of Chemistry and Bioengineering,Taizhou College,Nanjing Normal University,Taizhou 225300,China)
出处
《安徽化工》
CAS
2022年第1期28-31,共4页
Anhui Chemical Industry
基金
江苏省高等学校自然科学基金(20KJD180003)
江苏省高校实验室研究会立项课题(GS2019YB06)资助
2020年江苏省大学生创新创业训练计划项目(202013843017)。
关键词
纳米级二氧化钛
圆形盘肠溞
急性毒性
理化指数
nano titanium dioxide
Chydorussphaericus
acute toxicity
physical and chemical index
