摘要
基于超高效液相色谱-四级杆串联飞行时间质谱对都匀毛尖茶的大叶种(长椭圆形叶片棕色茎,BS)和小叶种(椭圆形叶片绿色茎,GS)进行代谢组学研究。结果表明,主成分分析可以明显区分开这两个茶树品种;正交偏最小二乘判别分析检测到7种显著差异代谢物,包括4种黄酮醇类物质(杨梅素、二氢槲皮素、槲皮素和山奈酚)、2种儿茶素(表儿茶素和表没食子儿茶素)、1种酚酸类物质咖啡酰奎尼酸。通路富集分析显示,这7种代谢物主要参与类黄酮生物合成途径。此外,表儿茶素和表没食子儿茶素在GS品种中含量较高,而杨梅素、二氢槲皮素、槲皮素、山奈酚和咖啡酰奎尼酸在BS品种中含量较高,这表明GS品种更适合绿茶的加工,BS品种更适合红茶的加工。
In this paper,the metabolomics of large leaf species(long oval leaf with brown stem,BS)and small leaf species(oval leaf with green stem,GS)of Duyun Maojian tea were studied based on Ultra performance liquid chromatography-quadrupole tandem time of flight mass spectrometry.The results showed that principal component analysis could distinguish the two tea varieties.Seven metabolites were detected by orthogonal partial least squares discriminant analysis,including four flavonols(myricetin,dihydroquercetin,quercetin and kaempferol),two catechins(epicatechin and epigallocatechin)and one phenolic acid(caffeoyl guinic acid).Further,pathway enrichment analysis showed that these seven metabolites were mainly involved in the flavonoid biosynthesis pathway.In addition,the contents of epicatechin and epigallocatechin were higher in GS variety,while the contents of myricetin,dihydroquercetin,quercetin,kaempferol and caffeoyl guinic acid were higher in BS variety,suggesting that GS variety was more suitable for the processing of green tea,and BS variety was more suitable for the processing of black tea.
作者
王春波
吕辉
韦玲冬
郭治友
WANG Chunbo;Lü Hui;WEI Lingdong;GUO Zhiyou(College of Biological Sciences and Agriculture,Qiannan Normal University for Nationalities,Duyun Guizhou 558000,China)
出处
《种子》
北大核心
2022年第1期44-48,共5页
Seed
基金
国家自然科学基金项目“贵州喀斯特山区野生茶树种群表观遗传分化中的环境效应研究”(31660056)
贵州省科技计划项目“都匀毛尖野生茶树代谢组学研究”(黔科合平台人[2020]QNSYXM02)。
关键词
都匀毛尖茶
代谢组学
代谢差异
茶叶加工
Duyun Maojian tea
metabolomics
metabolic difference
tea processing
