摘要
2017-2018年,从山东省8个大型集约化养鸡场中共采集720份新鲜粪便样品,通过细菌分离鉴定共分离到697株非重复大肠杆菌。采用琼脂稀释法对所有菌株进行药敏试验;利用PCR扩增多黏菌素耐药基因(mcr-1);并对mcr-1阳性菌株扩增β-内酰胺类耐药基因(ESBL)和喹诺酮类耐药基因(PMQR)。结果显示,从697株大肠杆菌中共检测到83株(11.9%)携带mcr-1基因,其中mcr-1阳性菌株对氨苄西林的耐药率最高(82/83,98.8%),其次是阿莫西林(81/83,97.6%)和氟苯尼考(79/83,95.2%),但对阿莫西林/克拉维酸的耐药率较低(18/83,21.7%)。从mcr-1阳性菌株中共检测到5种ESBL基因,其中所有菌株均携带bla;(83/83,100%),其次为bla;(67/83,80.7%);检测到4种PMQR基因,其中aac(6′)-Ib-cr检出率最高(34/83,40.9%),其次为qnrA(9/83,10.8%)。结果表明,山东省禽源大肠杆菌是mcr-1基因的储存库,可能对人类公共健康造成威胁,应继续加强对养鸡场中mcr-1耐药基因的监控。
This study aims to understand the prevalence of polymyxin-resistant gene mcr-1 in Escherichia coli(E.coli)from chicken farms in Shandong Province.From 2017 to 2018,a total of 720 fresh fecal samples were collected from 8 large-scale intensive chicken farms in Shandong Province,and 697 strains of non-repetitive E.coli were isolated through bacterial isolation and identification.Drug sensitivity test for all E.coli was performed using micro-broth dilution method.PCR was used to amplify the polymyxin resistant gene mcr-1 and mcr-1 positive isolates were PCR-screened forβ-lactam resistance gene(ESBL)and quinolone resistance gene(PMQR).The results showed that 83(11.9%)of 697 E.coli harboured the mcr-1 gene.The resistance rate of mcr-1 positive strains to ampicillin was the highest(82/83,98.8%),followed by amoxicillin(81/83,97.6%)and florfenicol(79/83,95.2%),however,the resistance rate to amoxicillin/clavulanic acid was low(18/83,21.7%).Five ESBL genes were detected from mcr-1 positive strains,of which bla;(83/83,100%)was carried by all strains,followed by bla;(67/83,80.7%);four PMQR genes were detected,among which aac(6′)-Ib-cr was the highest(34/83,40.9%),followed by qnrA(19/83,10.8%).In conclusion,E.coli of chicken in Shandong Province is the repository of mcr-1 gene,which may pose a threat to human public health.We should continue to strengthen the monitoring of mcr-1 resistance gene in chicken farms.
作者
张印
苑晓萌
李璐璐
张庆
胡明
齐静
骆延波
赵效南
刘玉庆
ZHANG Yin;YUAN Xiaomeng;LI Lulu;ZHANG Qing;HU Ming;QI Jing;LUO Yanbo;ZHAO Xiaonan;LIU Yuqing(Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2022年第1期66-70,共5页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(2019YFA0904004)
山东省重点科技创新工程资助项目(2019JZZY010719)
山东省农业科学院农业技术创新工程资助项目(CXGC2018E10)。
关键词
大肠杆菌
养鸡场
mcr-1
药敏试验
耐药基因
Escherichia coli
chicken farms
mcr-1
drug sensitivity test
resistance gene
