摘要
目的探讨长链非编码RNA(lncRNA)-H19对甲状腺癌细胞增殖、侵袭和细胞周期的影响。方法检测lncRNA-H19在癌组织、癌旁组织、正常人甲状腺上皮细胞TEC及甲状腺癌细胞KAT18、FTC133、BCPAP、TPC-1中的表达;检测沉默lncRNA-H19对细胞增殖、侵袭和细胞周期的影响。结果lncRNA-H19在甲状腺癌组织和细胞系中高表达;不同肿瘤大小、N分期、TNM分期患者的lncRNA-H19表达水平差异显著(P<0.05)。沉默lncRNA-H19后,48、72 h的细胞增殖率及克隆形成率、穿膜细胞数、微血管形成数量均明显下降(P<0.05);Ki67、PCNA、N-cadherin、Fibronectin、VEGF、MMP-9、MMP-14、cyclin A、CDK2、CDK4、cyclin D1蛋白表达明显下调(P<0.05),而E-cadherin蛋白表达明显上调(P<0.05)。结论沉默lncRNA-H19可能通过调节周期相关蛋白表达调节FTC133细胞增殖、侵袭及细胞周期。
Objective To explore the effects of long non-coding RNA(lncRNA)-H19 on the proliferation,invasion and cell cycle of thyroid cancer cells.Methods The expression of lncRNA-H19 in cancer tissues,para-cancerous tissues,normal human thyroid epithelial cells(TEC)and thyroid cancer cells(KAT18,FTC133,BCPAP,TPC-1)were detected.The effects of silencing lncRNA-H19 on cell proliferation,invasion and cell cycle were detected.Results The lncRNA-H19 was highly expressed in thyroid cancer tissues and cell lines.The expression levels of lncRNA-H19 among thyroid cancer patients with different tumor sizes,N staging and TNM staging were sig-nificantly different(P<0.05).After silencing lncRNA-H19,the cell proliferation rate,clone for-mation rate,number of migrating cells and number of microvessel formation at 48 h and 72 h were significantly decreased(P<0.05),the expression levels of Ki67,PCNA,N-cadherin,Fibronec-tin,VEGF,MMP-9,MMP-14,cyclin A,CDK2,CDK4 and cyclin D1 proteins were significant-ly decreased(P<0.05),and the expression level of E-cadherin protein was significantly increased(P<0.05).Conclusion Silencing lncRNA-H19 could regulate the proliferation,invasion and cell cycle of FTC133 by regulating the expression of cell cycle related proteins.
作者
文霆
邵建斌
方因
蔡宗龙
WEN Ting;SHAO Jianbin;FANG Yin;CAI Zonglong(Department of General Surgery,The First Affiliated Hospital of Xiamen University,Xiamen,Fujian,361003,China)
出处
《医学分子生物学杂志》
CAS
2022年第1期26-32,共7页
Journal of Medical Molecular Biology
基金
福建省自然科学基金(No.2020J05208)。
