摘要
为制备羊源解淀粉芽孢杆菌fsznc-06菌.剂,采用响应面法,分析优化冻干保护剂配方,从离心条件、保护剂筛选与复配、稳定性检测等方面探究对菌体存活率的影响.结果表明,最佳离心条件为6000 r/min离心10 min,优化后的冻干保护剂配方为丙三醇(3.102 g/100 mL)、蔗糖(2.926 g/100 mL)和葡萄糖(7.096 g/100 mL),在该条件下解淀粉芽孢杆菌存活率达到96.47%,与理论预测值接近.冻干菌剂在4℃条件下贮存稳定性更高,保藏180天后的存活率约为66.30%,说明响应面法可用于优化解淀粉芽孢杆菌冻干保护剂.
To prepare Bacillus amyloliquefaciens fsznc-06 from goat feces,cryoprotectants were optimized by response surface analysis.The effects on the survival rate of bacteria were explored from the aspects of centrifugal conditions,selection,formulation of cryoprotectants and stability detection.The results showed that the optimal centrifugation conditions were 6000 r/min for 10 min.The optimized cryoprotectants contained glycerol(3.102 g/100 mL),sucrose(2.926 g/100 mL),and glucose(7.096 g/100 mL),and its lyophilization survival rate reached 96.47%,which was close to the theoretical prediction.The freeze-dried bacterial agent had higher storage stability at 4℃,and the survival rate after storage for 180 days was about 66.30%.Therefore the response surface method can be used to optimize the cryoprotectant of Bacillus amyloliquefaciens.
作者
张玲
张楠驰
魏勇
许锋
张林
王利
俄木曲者
ZHANG Ling;ZHANG Nan-chi;WEI Yong;XU Feng;ZHANG Lin;WANG Li;E-MU Qu-zhe(Key Laboratory of Animal Science of State Ethnic Affairs Commission,Southwest Minzu University,Chengdu 610041,China;Aba Vocational College,Maoxian 623200,China;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Animal Science Academy of Sichuan Province,Chengdu 610066,China)
出处
《西南民族大学学报（自然科学版）》
CAS
2022年第1期22-28,共7页
Journal of Southwest Minzu University(Natural Science Edition)
基金
四川省科技厅项目(2021YFN007,2020NZZJ030)
四川省肉羊创新团队防疫岗位资助项目(sccxtd-2020-14)。
关键词
羊源解淀粉芽孢杆菌
响应面分析法
冻干保护剂
Bacillus amyloliquefaciens
goat
response surface methodology
cryoprotectant
