摘要
手性超分子水凝胶能够仿生细胞外手性微环境,在组织工程中具有特殊的意义,但其强度和稳定性较低,仍然面临着巨大的挑战。本文将无机羟基磷灰石纳米颗粒(HAP)引入到苯丙氨酸衍生物手性超分子水凝胶(LPF)中以改善其力学性能和生物功能。圆二色光谱和扫描电子显微镜结果显示,HAP掺入后LPF组装手性发生反转。与纯LPF水凝胶相比,杂化水凝胶具有优异的力学性能和长期稳定性。LPF储能模量G’值仅为452 Pa,在24 h内迅速崩塌;而LPF/HAP水凝胶的G’提高到3353 Pa,保持凝胶态时间超过3个月。HAP纳米粒子表面上丰富的Ca2+离子与LPF羧基形成的配位作用是水凝胶强化的关键因素。此外,HAP加入还提高了对细胞的成骨刺激,表现为LPF/HAP基质上成骨细胞碱性磷酸酶(ALP)活性的增加,这不仅因为羟基磷灰石的骨诱导能力,还归因于HAP使纳米纤维的刚度和稳定性明显改善。通过无机纳米颗粒的力学增强和功能化,手性超分子水凝胶在骨组织工程中具有广阔的应用前景。
Chiral supramolecular hydrogels that can mimic the chiral extracellular microenvironment are of special significance in tissue engineering,however,it still remains a great challenge for their low long lifetime stability.Herein,we introduce inorganic hydroxyapatite nanoparticles(HAP)to the well-established phenylalanine derivative chiral supramolecular hydrogel(LPF),aiming to improve its mechanical performance as well as biofunctions.Morphological studies revealed an inversion of the chirality of LPF after HAP incorporation.Rheological experiments confirmed the long-term stability and superior mechanical property of the hybrid hydrogel over the neat hydrogel,where LPF/HAP hydrogel exhibited an improved elastic modulus of 3353 Pa,standing over three months,and LPF hydrogel collapsed soon within 24 hours with a much lower storage modulus(G’)value of 452 Pa.The abundant coordination interactions supplied by Ca;ions on the surface of HAP nanoparticles crosslinking with the carboxyl group are considered to be the crucial factors of the mechanical strengthening.Moreover,the HAP incorporation led to an enhanced stimulation of the osteogenesis of the osteoblastic cells,demonstrating by the increasement of the alkaline phosphatase(ALP)activity on LPF/HAP substrate,which ascribed not only to the osteoinductive ability of hydroxyapatite,but also to the improved stiffness and stability induced by HAP.The findings indicate that the chiral supramolecular hydrogel is promising in bone tissue engineering via inorganic nanoparticle strengthening and functionalization.
作者
赵常利
秦明高
窦晓秋
冯传良
ZHAO Chang-Li;QIN Ming-Gao;DOU Xiao-Qiu;FENG Chuan-Liang(State Key Laboratory of Metal Matrix Composites,School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《应用化学》
CAS
CSCD
北大核心
2022年第1期177-187,共11页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(No.51833006)
上海市自然基金(No.19ZR1425400)
上海市科学技术委员会科技创新行动计划项目(No.19441903000)资助。
关键词
超分子水凝胶
无机纳米颗粒
杂化
力学稳定性
成骨
手性
Supramolecular hydrogel
Inorganic nanoparticle
Hybrid
Mechanical stability
Osteogenesis
Chirality
