摘要
目的研究黄蜀葵花不同开放程度对黄蜀葵花药材中总黄酮、金丝桃苷、异槲皮苷含量及其醇提取物的影响。方法采用紫外-可见分光光度法测定黄蜀葵花药材中总黄酮含量,采用高效液相色谱法测定黄蜀葵花药材中金丝桃苷和异槲皮苷的含量,并比较黄蜀葵花药材醇提转移率,评价不同开放程度样品之间的差异。结果完全开放的黄色黄蜀葵花中各成分含量均较高,醇提转移率高、油层较少,半开放及未开放黄绿色的黄蜀葵花中各成分均偏低,醇提转移率较低、油层较多。结论黄蜀葵花药材宜在花期内完全开放后采收,颜色以亮黄色为最佳,本研究为各地黄蜀葵花药材的进一步开发利用提供了质量控制依据。
Objective To research the effects of different flowering degrees of Abelmoschus manihot(L.)Medic on the contents of total flavonoids,hyperoside,isoquercitrin and the ethanol extracts.Methods The content of total flavonoids in A.manihot(L.)Medic was determined by UV-Vis spectrophotometry,and the contents of hyperoside and isoquercitrin were determined by HPLC.The ethanol extraction transfer rates of A.manihot(L.)Medic were compared to evaluate the differences among samples with different flowing degrees.Results The content of each component in fully-opened-yellow flower was higher,the alcohol extraction transfer rate was higher and the oil layer was less.The content of each component in semi-opened and unopened yellow-green flower was lower,the alcohol extraction transfer rate was lower and the oil layer was more than others.Conclusion The A.manihot should be harvested after full opening during the flowering period,and bright yellow is the best color.This study provided quality control basis for the further development and utilization of A.manihot in various regions.
作者
郭小藤
刘燕
张清华
尚立霞
GUO Xiaoteng;LIU Yan;ZHANG Qinghua;SHANG Lixia(Key Laboratory of Taishan Scholar,Shandong Academy of Pharmaceutical Science,Jinan 250101,China;Shandong Provincial Key Laboratory of Biopharmaceuticals,Shandong Academy of Pharmaceutical Science,Jinan 250101,China)
出处
《药学研究》
CAS
2022年第1期26-30,共5页
Journal of Pharmaceutical Research
基金
药物分析与质量控制中心创新能力建设项目(山东省企业重大技术创新及产业化项目)。
关键词
黄蜀葵花
不同开放程度
总黄酮
金丝桃苷
异槲皮苷
提取率
Abelmoschus manihot L.Medic
Different flowering degree
Total flavonoids
Hyperoside
Isoquercitrin
Extraction rate
