摘要
该研究探讨长链非编码RNA(LncRNA)LINC02310对非小细胞肺癌上皮–间质转化(EMT)和放射敏感性的影响。采用实时定量聚合酶链反应(qRT-PCR)法检测非小细胞肺癌组织和细胞(H1299、A549、H1650)内LINC02310相对表达量。体外培养H1299细胞,按照脂质体法将si-NC、si-LINC02310转染至细胞内,记为si-NC组、si-LINC02310组,采用qRT-PCR法检测LINC02310相对表达量;噻唑蓝(MTT)法检测细胞增殖;流式细胞术检测细胞凋亡;蛋白免疫印迹(Western blot)法检测Bcl-2相关X蛋白(Bax)、B细胞淋巴瘤/白血病-2(Bcl2)、蜗牛蛋白(Snail)、E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)蛋白表达;Transwell实验检测细胞迁移和侵袭;克隆形成实验检测放射敏感性。与癌旁组织和正常人支气管上皮细胞HBE比较,LINC02310在非小细胞肺癌组织和细胞中相对表达量均明显升高。与si-NC组比较,下调LINC02310能降低非小细胞肺癌细胞的D值、迁移细胞数、侵袭细胞数、细胞存活分数,增加凋亡率,降低Bcl2、Snail、N-cadherin蛋白表达,增加Bax、E-cadherin蛋白表达。下调LINC02310能抑制非小细胞肺癌细胞增殖、迁移、侵袭和上皮–间质转化,促进细胞凋亡,增强放射敏感性。
This study was to investigate the effects of LncRNA(long non-coding RNA)LINC02310 on EMT(epithelial-mesenchymal transition)and radiosensitivity of non-small cell lung cancer.qRT-PCR(real-time quantitative polymerase chain reaction)method was used to detect the relative expression of LINC02310 in non-small cell lung cancer tissues and cells(H1299,A549,H1650).H1299 cells were cultured in vitro,si-NC and si-LINC02310 were transfected into the cells according to the liposome method,which were marked as si-NC group and si-LINC02310 group,and the relative expression of LINC02310 was detected by qRT-PCR;thiazole blue(MTT)method was used to detect cell proliferation;flow cytometry was used to detect cell apoptosis;Western blot method was used to detect Bax(Bcl-2 related X protein),Bcl2(B-cell lymphoma/leukemia-2),Snail,E-cadherin,and N-cadherin protein expression;Transwell test was used to detect cell migration and invasion;clone formation test was used to detect radiosensitivity.Compared with adjacent tissues and normal human lung epithelial cells HBE,the relative expression of LINC02310 in non-small cell lung cancer tissues and cells was significantly increased.Compared with the si-NC group,down-regulating LINC02310 can reduce the D value,the number of migrated cells,the number of invaded cells,and the cell SF(survival fraction)of non-small cell lung cancer cells,increase the rate of apoptosis;reduce the expression of Bcl2,Snail,and N-cadherin,and increase the expression of Bax and E-cadherin.Downregulation of LINC02310 can inhibit non-small cell lung cancer cell proliferation,migration,invasion and epithelial-mesenchymal transition,promote cell apoptosis and increase radiosensitivity.
作者
吴朝晖
杨建胜
林贤宾
吴佳云
吴敬阳
WU Chaohui;YANG Jiansheng;LIN Xianbin;WU Jiayun;WU Jingyang(Department of Thoracic and Cardiovascular Surgery,the Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2021年第12期2361-2368,共8页
Chinese Journal of Cell Biology
基金
泉州市科技计划项目(批准号:2019N101S)资助的课题。
