摘要
目的探讨血清miR-21和miR-119a是否可以预测膀胱肿瘤并分析其与肿瘤恶性增殖及侵袭的关系。方法膀胱癌患者及健康体检者各48例,分为膀胱癌组和正常对照组。荧光定量PCR法检测血清和组织中miR-21和miR-119a的表达水平。蛋白质印迹法检测PTEN,PI3K,AKT,CyclinD1,TGF-β,β-Catenin,N-cadherin和E-cadherin的表达。流式细胞术检测细胞凋亡率和周期分布。Perason相关分析血清miR-21和miR-119a表达与侵袭性基因的相关性。结果与正常对照组比较,膀胱癌组血清miR-21和miR-119a相对表达量均升高(均P<0.05);血清miR-21和miR-119a对膀胱癌的分型具有良好的鉴别能力,其AUC分别为0.995和0.996。与癌旁正常组织比较,膀胱癌组织miR-21和miR-119a相对表达量均升高(均P<0.05),PTEN和E-cadheri相对表达量降低(均P<0.05),PI3K、AKT、CyclinD1、TGF-β、β-catenin和N-cadherin相对表达量均升高(均P<0.05)。与膀胱癌正常组织比较,膀胱癌组织凋亡率较低,膀胱癌组织G1期的比例低于膀胱癌正常组织,S、G2期的比例则高于膀胱癌正常组织(均P<0.05);Pearson相关分析显示,血清miR-21和miR-119a表达水平与PI3K、AKT、CyclinD1、TGF-β、β-catenin及Ncadherin表达呈正相关(均P<0.05),与PTEN和E-cadherin表达呈负相关(均P<0.05)。结论血清中miR-21和miR-119a的表达水平对膀胱癌的预测具有较高的价值,膀胱癌患者血清和癌组织中miR-21、miR-119a表达水平均升高,且血清miR-21、miR-119a与增殖和侵袭相关基因相关,提示血清miR-21、miR-119a可能是膀胱癌的潜在生物标志物。
Objective To explore whether the serum miR-21 and mir-119a could predict bladder cancer and to analyze their association with tumor malignant proliferation and invasion.Methods Bladder cancer patients and healthy 48 cases in each group.The expression levels of miR-21 and mir-119a in serum and tissues were detected by fluores‐cence quantitative PCR.The expressions of PTEN,PI3K,Akt,CyclinD1,TGF-β,β-Catenin,N-cadherin and E-cad‐herin were detected by Western blot.Apoptosis rate and cycle distribution were detected by flow cytometry.Perason cor‐relation was used to analyze the correlation between the two miRNAs in serum and the invasive genes.Results Com‐pared to the normal group,the relative expression of serum miR-21 and miR-119a were both increased in bladder cancer group(both P values<0.05).MiR-21 and miR-119a in serum showed good discrimination power in classification of bladder cancer and normal with AUC 0.995 and 0.996 respectively.Compared to the adjacent normal tissues,the rela‐tive expressions of miR21 and miR-119a were increased(both P values<0.05),and the relative expressions of PTEN and E-cadheri were decreased in bladder cancer tissues(both P values<0.05)while the relative expressions of PI3K,AKT,CyclinD1,TGF-β,β-catenin and N cadherin were increased(all P values<0.05).Compared to the adjacent normal tissues,the apoptosis rate of bladder cancer tissues was lower.The proportion of bladder cancer tissues in G1 stage was lower than that of in the adjacent normal tissues,while the proportion of S and G2 stage was higher(P values<0.05).Pearson correlation analysis showed that serum miR-21 and miR-119a was positively correlated with PI3K,AKT,Cy‐clinD1,TGF-β,β-catenin and N-cadherin(all P<0.05),and negatively correlated with PTEN and E-cadherin in the can‐cer tissue(all P values<0.05).Conclusion MiR-21 and mir-119a in serum had a wonderful power in predicting bladder cancer.The expression levels of the two miRNAs were increased in both serum and cancer tissue of the bladder cancer patients.MiR-21 and mir-119a in serum were correlated with the proliferation and invasive related genes.MiR-21 and mir-119a in serum could be potential biomarker of bladder cancer.
作者
吴德柱
李清林
彭娜
林建峰
WU Dezhu;LI Qinglin;PENG Na;LIN Jianfeng(Department of Urology,Putian Medical District,No.900 Hospital of Joint Logistics Support Force,Putian Fujian 351100,China)
出处
《转化医学杂志》
2022年第1期9-13,8,共6页
Translational Medicine Journal
