摘要
SQUAMOSA promoter binding protein-like(SPL)基因家族在植物成花转变与花器发育中起重要调控作用。该研究基于甜橙(Citrus sinensis)基因组数据,对甜橙SPL家族成员及其启动子区进行生物信息学分析,并采用qRT-PCR检测其在甜橙不同花期、不同品种花芽和叶片的表达特异性,为深入探讨CsSPLs基因在甜橙成花诱导中的功能奠定基础。结果表明:(1)共鉴定出15个含有SBP保守结构域的甜橙SPL基因,分别命名为CsSPL1~CsSPL15,且分布在6条染色体上。(2)CsSPL1~CsSPL15基因编码的氨基酸长度为130~1075 aa,分子量为14.73~118.75 kD;系统发育分析将15个CsSPLs分成8个亚族,除CsSPL4外,其他CsSPLs均与拟南芥SPL家族成员聚类。(3)顺式作用元件分析表明,CsSPLs启动子中含有大量光响应元件,推测CsSPLs可能在甜橙响应光调控过程中发挥重要作用。(4)转录组数据分析显示,CsSPLs在甜橙愈伤组织、花、叶片和果实中均有表达,其中CsSPL3、CsSPL4、CsSPL7、CsSPL8、CsSPL12、CsSPL13、CsSPL14和CsSPL15在花和叶片中较高表达。(5)qRT-PCR检测显示,CsSPLs在甜橙不同花期、不同品种花芽和叶片组织中的表达均有显著上调趋势,且采样各时期下早花品种‘赣南早’花芽和叶片中CsSPLs的表达量均高于对照品种‘纽荷尔’。研究认为,甜橙成花组织内CsSPLs基因的高表达是其花期提前的主要因素。
SQUAMOSA promoter-binding protein like(SPL)gene family plays an important regulatory role in floral transition and flower development.In this study,we used bioinformatics method to identify 15 CsSPLs with SBP conserved domain from the whole genome of Citrus sinensis,and the relative expression specificity in flower buds and leaves in C.sinensis of different flowering and varieties by using qRT-PCR method,which laid a foundation for further exploring the function of CsSPLs during flowering induction.The results show that:(1)these genes distributed on 6 chromosomes and they were named CsSPL1-CsSPL15.(2)Analysis of physicochemical properties showed that the length of the CsSPLs proteins ranged from 130 to 1075 aa with molecular mass of 14.73 to 118.75 kD.Phylogenetic analysis showed that 15 CsSPLs were divided into 8 subfamilies,and most CsSPLs were clustered with AtSPLs,except for CsSPL4.(3)The predictive analysis of cis-acting elements showed that CsSPLs promoter contained a large number of light responsiveness elements,indicating that they might play an important role in the regulation of C.sinensis.(4)Transcriptome data analysis showed that CsSPLs expressed in callus,flowers,leaves and fruits of C.sinensis.The expression of CsSPL3,CsSPL4,CsSPL7,CsSPL8,CsSPL12,CsSPL13,CsSPL14 and CsSPL15 were high in flowers and leaves.(5)The qRT-PCR results showed that the relative expression levels of CsSPLs were significantly up-regulated in flower buds and leaves of two C.sinensis varieties.And CsSPLs in‘Gannan Zao’cultivars were higher than that in flower buds and leaves of‘Newhall’cultivars at 5 sampling time points.These results showed that the high expression of CsSPLs in flower buds and leaves led to the early flowering of C.sinensis.
作者
杨杰
陈蓉
胡文娟
吴巧玲
陈健美
李兴涛
YANG Jie;CHEN Rong;HU Wenjuan;WU Qiaoling;CHEN Jianmei;LI Xingtao(College of Life Sciences, Gannan Normal University, National Navel Orange Engineering Research Center, Ganzhou, Jiangxi 341000, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第1期48-56,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(32160731)
江西省教育厅科学技术研究项目(GJJ190758,GJJ180758)。
关键词
甜橙
SPL基因家族
生物信息学
基因表达
成花诱导
Citrus sinensis
SPL gene family
bioinformatics
genic expression
flowering induction
