摘要
目的探讨脾虚湿困型肥胖症患者肠道菌群多样性差异及相关性。方法招募59例脾虚湿困型肥胖症患者,按糖化血红蛋白A1c(HbA1c)分为肥胖1(FAT1)组35例(HbA1c<6%)及肥胖2(FAT2)组24例(HbA1c 6%),并招募29名健康志愿者为对照组。获取肥胖组及对照组新鲜粪便,提取肠道菌群总DNA,采用Illumina Miseq高通量测序平台,对粪便样本中所有细菌的16S rRNA V3-V4区进行DNA测序,对测序结果进行数据分析。结果脾虚湿困型肥胖症患者存在肠道菌群失调,其肠道微生物的丰富度与多样性均上升。粪杆菌属(Faecalibacterium)、布劳特氏菌属(Blautia)、罕见小球菌属(Subdoligranulum)及小类杆菌属(Dialister)肥胖组比例低于对照组(对照组>FAT1组>FAT2组,P<0.05),巨单胞菌属(Megamonas)(对照组<FAT1组<FAT2组,P<0.05)和乳杆菌属(Lactobacillus)(对照组<FAT2组<FAT1组,P<0.05)肥胖组比例高于对照组。环境因子关联分析提示脾虚湿困型肥胖症患者菌群分布与HbA1c、空腹血糖、餐后血糖相关。结论脾虚湿困型肥胖症患者肠道菌群的数量、种类和分布均存在异常,其肠道菌群丰富度与多样性上升,HbA1c及血糖水平在其肠道菌群分布中发挥重要作用。
Objective To observe the distribution diversities and correlations of gut microbiota in obesity patients with Pi-deficiency induced dampness and drawsiness syndrome(PDIDDS).Methods Totally 59 obesity patients with PDIDDS were enrolled according to HbA1c,35 in FAT1 group(HbA1c<6%)and 24 in FAT2 group(HbA1c 6%),and 29 healthy volunteers were also enrolled as the control(CON).Fresh feces were collected from obesity patients and healthy volunteers,and the total DNA of intestinal flora was extracted.Illumina Miseq high-throughput sequencing platform was used to conduct DNA sequencing in the V3-V4 region of 16S rRNA of all the bacteria in feces samples,and the sequencing results were analyzed.Results Obesity patients with PDIDDS had imbalanced gut microbiota,with increased richness and diversities.Ratios of Faecalibacterium,Blautia,Subdoligranulum and Dialister were lower in obesity patients than in the control group(CON>FAT1>FAT2,P<0.05).Ratios of Megamonas(CON<FAT1<FAT2,P<0.05)and Lactobacillus(CON<FAT2<FAT1,P<0.05)were higher in obesity patients than in the control group.The correlation analyses of environmental factors suggested that the flora distributions were correlated with HbA1c,fasting blood glucose(FBG)and postprandial blood glucose(PBG)in obesity patients with PDIDDS.Conclusions The quantities,species,and distributions of gut microbiota were abnormal in obesity patients with PDIDDS,with increased richness and diversities.HbA1c and blood levels play important roles in the distribution of gut microbiota.
作者
何珂
陆西宛
朱丽华
朱立萍
HE Ke;LU Xi-wan;ZHU Li-hua;ZHU Li-ping(Department of Endocrinology,Wuxi Hospital Affiliated to Nanjing University of Chinese Medicine,Jiangsu(214000))
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2022年第1期40-48,共9页
Chinese Journal of Integrated Traditional and Western Medicine
基金
江苏省中医药局科技项目(No.YB2017048)
无锡市卫健委面上项目(No.M202008)
无锡市卫健委青年项目(No.Q202151)
无锡市双百后备拔尖人才项目(No.HB2020067)
无锡市中医医院院内重点项目(No.ZYYZD21005)。
关键词
肥胖
脾虚湿困
肠道菌群
多样性
obesity
Pi-deficiency induced dampness and drawsiness
gut microbiota
diversity
