摘要
目的探讨酪氨酸激酶受体B(TrkB)抑制剂对前列腺癌细胞生物学效应及血管内皮生长因子(VEGF)表达的影响。方法将培养后的人前列腺癌PC3细胞分为空白对照组、酪氨酸激酶抑制剂(K252a)干预组,K252a干预组又分为150 nmol/L、300 nmol/L、450 nmol/L、600 nmol/L的4个亚组,采用四甲基偶氮唑蓝(MTT)法检测K252a对前列腺癌PC3细胞的细胞增殖抑制率;选择450 nmol/L K252a作为干预组与空白对照组比较,采用Transwell法检测2组前列腺癌PC3细胞的迁移数目,流式细胞术检测2组前列腺癌PC3细胞周期分布百分比和细胞凋亡率,Western blot检测2组前列腺癌PC3细胞中TrkB、VEGF蛋白的表达。结果4个不同浓度K252a的作用下,随着作用时间的延长,前列腺癌PC3细胞的增殖抑制率呈整体上升趋势,均高于空白组(P<0.05);K252a干预组PC3细胞24 h的迁移数目低于空白对照组(P<0.05),K252a干预组的G1期细胞比率高于空白对照组(P<0.05),K252a干预组的S期细胞比率低于空白对照组(P<0.05),K252a干预组的细胞凋亡率高于空白对照组(P<0.05),K252a干预组的TrkB、VEGF蛋白相对表达量均低于空白对照组(P<0.05)。结论K252a对前列腺癌PC3细胞增殖、迁移和生长具有抑制作用,其机制可能与K252a抑制前列腺癌细胞TrkB及VEGF蛋白的表达有关。
Objective To investigate the effect of tyrosine kinase receptor B(TrkB)inhibitor on the biology and vascular endothelial growth factor(VEGF)expression of prostate cancer cells.Methods Cultured PC3 cells were divided into blank control and TrkB inhibitor(K252a)groups.K252a group was further divided into 4 subgroups:150 nmol/L,300 nmol/L,450 nmol/L,and 600 nmol/L.Methyl Thiazolyl Tetrazolium(MTT)assay was applied to measure the inhibitory rate of K252a on cell proliferation.Subgroup 450 nmol/L and blank control group were selected for the following study.Transwell assay was used to detect the migratory number of PC3 cells in two groups.Flow cytometry was used to detect the cell cycle distribution and cellular apoptotic rates of two groups.Western blot was used to detect the expression of TrkB and VEGF protein in two groups.Results Inhibitory rates of K252a in cell proliferation at 4 different concentration were increased in a time-dependent manner,higher than that of blank control group(P<0.05).The number of migratory cells in K252a group at 24 hours was significantly lower than that in blank control group(P<0.05).The percentage of cells at G1 phase was higher in K252a group than that in blank control group(P<0.05),but the percentage of cells at S phase was lower in K252a group than that in blank control group(P<0.05).The apoptotic rate of K252a group was significantly greater than that of blank control group(P<0.05),and the relative protein expression of TrkB and VEGF in K252a group were less than that of blank control group(P<0.05).Conclusion K252a has an inhibitory effect on cell proliferation,migration and growth of PC3 cells.The mechanism may be related to the inhibition of K252a on the expression of TrkB and VEGF protein in prostate cancer cells.
作者
张帆
汪锋
钗丽干
都吉雅
崔宏伟
苏依图
ZHANG Fan;WANG Feng;CHAI Ligan;DU Jiya;CUI Hongwei;SU Yitu(Department of Pathology,the People's Hospital of Inner Mongolia Autonomous Region,Hohhot 010017,Inner Mongolia,China;Department of Clinical Medical Research Center,the People's Hospital of Inner Mongolia Autonomous Region,Hohhot 010017,Inner Mongolia,China)
出处
《贵州医科大学学报》
CAS
2022年第2期203-208,共6页
Journal of Guizhou Medical University
基金
内蒙古自治区自然科学基金项目(2018MS08082)
内蒙古医科大学科技百万工程联合项目(YKD2018KJBW〔LH〕064)。
