摘要
以龙眼(Dimocarpus longan Lour.)‘红核子’品种早期体细胞胚胎发生的胚性愈伤组织(embryogenic callus,EC)、不完全胚性紧实结构(incomplete embryogenic compact structures,ICpEC)和球形胚(globular embryos,GE)为材料,通过生物信息学方法鉴定和分析其染色质重塑因子DlSnf2家族成员及分子进化特性,并通过转录组和实时荧光定量PCR (real-time quantitative PCR,qRT-PCR)分析其表达模式。龙眼DlSnf2家族包含35个成员,家族扩张受到全基因组或片段复制驱动。选择含有SNF2 N和HELICc结构域的31个成员,根据进化特征分为6大类19个亚族。蛋白互作、microRNA和启动子预测显示,部分成员能够发生互作,大部分基因受到microRNA的潜在调控,且存在与激素、胁迫、光、生长和发育相关顺式作用元件。转录组和qRT-PCR分析显示:大多数基因在ICpEC阶段中高表达,DlCHR18、DlCHR24、DlCHR25、DlCHR29和DlCHR31a从EC到GE的过程中表达逐渐下调,而DlCHR4、DlCHR15和DlCHR42表达逐渐上调。分析显示DlSnf2家族基因响应2,4-D、ABA、乙烯利、茉莉酸甲酯、4℃和40℃。DlCHR10和DlCHR11的表达被乙烯利上调,而DlCHR28b和DlCHR36被下调,DlCHR11被ABA和高温(40℃)下调,DlCHR10被茉莉酸甲酯下调,DlCHR36被低温(4℃)下调,DlCHR24和DlCHR35被高温(40℃)下调。Snf2基因家族在植物进化过程中可能具有功能的相对保守性及一定物种特异性。在植物生长调节剂和温度处理下,龙眼DlSnf2家族成员可能参与调控体胚发生和抵御高、低温胁迫,且推测DNA甲基化、microRNA和组蛋白修饰等其他表观调控机制参与了这一过程。
EC(embryogenic callus),ICpEC(incomplete embryogenic compact structures)and GE(globular embryos)of the early somatic embryogenesis(SE)of Dimocarpus longan Lour.‘Honghezi’were used as experimental materials. Using bioinformatic methods identified and analyzed the members and its molecular evolutionary characteristic of longan chromatin remodeling factor DlSnf2 family,and the expression patterns of DlSnf2 gene family were analyzed through transcriptome data and real-time quantitative PCR(qRT-PCR). The longan DlSnf2 family contains 35 members,and expansion of the family is mainly driven by whole-genomic or segmental duplication events. 31 members of containing SNF2_N and HELICc domains were selected and divided into 19 subfamilies under six categories depending on the evolutionary characteristics. Protein interaction,microRNA and promoter prediction showed that partial members can interact each other,almost all genes may be potentially regulated by microRNA and exist a large number of cis-acting elements related to hormone,stress,light,growth and development;Transcriptome and qRT-PCR analysis showed that most genes were highly expressed in ICpEC,and the expressions of DlCHR18,DlCHR24,DlCHR25,DlCHR29 and DlCHR31 a were gradually decreased from EC to GE,while DlCHR4,DlCHR15 and DlCHR42 gradually increased;The analysis exhibited that DlSnf2 family genes responded to 2,4-D,ABA,ethrel,Methyl jasmonate,4 ℃ and 40 ℃. Expressions of DlCHR10 and DlCHR11 are up regulated by ethrel,while DlCHR28 b and DlCHR36 are down regulated. DlCHR11 is down regulated by ABA and high temperature,DlCHR10 is down regulated by Methyl Jasmonate,DlCHR36 is down regulated by low temperature,and DlCHR24 and DlCHR35 are down regulated by high temperature. The Snf2 gene family may have relatively conservative functions and certain species specificity during plant evolution. Under plant growth regulator and temperature treatments,longan DlSnf2 family members may be involved in regulating somatic embryogenesis and resisting high and low temperature stress,and it is speculated that other epigenetic regulatory mechanisms such as DNA methylation,microRNA and histone modification are involved in the process.
作者
申序
陈晓慧
张婧
陈荣珠
徐小萍
李晓斐
蒋梦琦
刘蒲东
倪珊珊
林玉玲
赖钟雄
SHEN Xu;CHEN Xiaohui;ZHANG Jing;CHEN Rongzhu;XU Xiaoping;LI Xiaofei;JIANG Mengqi;LIU Pudong;NI Shanshan;LIN Yuling;LAI Zhongxiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第1期41-61,共21页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31572088,31672127)
福建省高原学科建设经费项目(102/71201801101)
福建农林大学科技创新基金项目(CXZX2020027A)。
关键词
龙眼
染色质重塑因子Snf2
进化
体胚发生
表观调控
Dimocarpus longan
chromatin remodeling factor Snf2
evolution
somatic embryogenesis
epigenetic regulation
