摘要
微生物诱导碳酸钙沉积(microbial induced calcium carbonate precipitation,MICP)是微生物通过新陈代谢在其周围微环境中形成碳酸钙沉积的一种自然现象,根据其原理目前研发出了“微生物水泥”。因其具有绿色环保、经济高效的特点,已成为生物、土木、环境等领域的研究热点。文中就与微生物水泥密切相关的关键酶——脲酶、碳酸酐酶的研究进展进行了系统综述,主要包括两种酶的基因、蛋白结构、调控机制、工程菌的构建以及脲酶与碳酸酐酶在MICP过程中的协同关系等,并对MICP未来的发展作出展望。在现有研究基础上,借助生物信息学、合成生物学等前沿技术,开发能适应广泛环境、高性能的生物水泥,这将开启MICP研究的一个新阶段。
Microbial induced calcium carbonate precipitation(MICP)refers to the natural biological process of calcium carbonate precipitation induced by microbial metabolism in its surrounding environment.Based on the principles of MICP,microbial cement has been developed and has received widespread attention in the field of biology,civil engineering,and environment owing to the merits of environmental friendliness and economic competence.Urease and carbonic anhydrase are the key enzymes closely related to microbial cement.This review summarizes the genes,protein structures,regulatory mechanisms,engineering strains and mutual synergistic relationship of these two enzymes.The application of bioinformatics and synthetic biology is expected to develop biocement with a wide range of environmental adaptability and high performance,and will bring the MICP research to a new height.
作者
王磊
王绪霞
李斐
崔明娟
杨晓旭
杨敏
闫云君
WANG Lei;WANG Xuxia;LI Fei;CUI Mingjuan;YANG Xiaoxu;YANG Min;YAN Yunjun(School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China;School of Chemical Engineering&Food Science,Zhengzhou University of Technology,Zhengzhou 450044,He’nan,China;School of Urban Construction,Wuchang Shouyi University,Wuhan 430064,Hubei,China;School of Civil Engineering and Mechanics,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2022年第2期506-517,共12页
Chinese Journal of Biotechnology
基金
中国博士后科学基金(2019M652622)
郑州工程技术学院科研基金(zggk202101)。
关键词
微生物诱导碳酸钙沉积
微生物水泥
酶
脲酶
碳酸酐酶
microbial induced calcium carbonate precipitation(MICP)
microbial cement
enzyme
urease
carbonic anhydrase
