摘要
目的:采用生物信息学的方法探讨影响前列腺癌放疗敏感性的差异基因表达及其生物学功能富集与疾病预后的潜在关系。方法:检索并获取GEO数据库中的关于前列腺癌放疗敏感性差异基因表达的数据(GSM3954350、GSM3954351、GSM3954352),GER2工具对差异基因进行筛选和分析,采用Enrichr数据库进行GO、KEGG富集分析,用Cytoscape软件构建蛋白质互作网络(protein-protein interaction,PPI),分析枢纽基因。结果:共筛选7041个差异表达的基因,其中3842个高表达基因,3199个低表达基因,选取了差异表达前20的基因进一步分析,它们的生物学功能主要富集在以下几个方面:核酸酶活性的调节、酶联受体蛋白信号通路、核苷酸切除修复、DNA缺口填补;细胞学组分主要定位于细胞外;分子功能富集于结构分子活性、受体结合、类丝氨酸肽酶活性等;KEGG信号通路分析显示差异基因主要富集于错配修复通路、非同源的末端结合通路等。结论:通过对前列腺癌放疗敏感性相关差异基因的分析,本研究发现的关键差异基因有助于了解前列腺癌发生放疗抵抗潜在的分子机制,可为进一步提高前列腺癌的放射治疗有效性提供理论依据。
Objective:To explore the potential relationship between differential gene expression,biological function enrichment and disease prognosis affecting the sensitivity of prostate cancer radiotherapy using bioinformatics.Methods:Retrieve and obtain data on differential gene expression of prostate cancer radiosensitivity in the GEO database(GSM3954350,GSM3954351,GSM3954352).GER2 tool was used to screen and analyze the differential genes.Enrichr database performed enrichment analysis of GO and KEGG.Cytoscape software built protein-protein interaction(PPI)networks and analyzed key genes.Results:A total of 7041 differentially expressed genes were screened,including 3842 high expression genes and 3199 low expression genes.The top 20 differentially expressed genes were selected for further analysis.Their biological functions were mainly enriched in the following aspects:Nuclease activity regulation,enzyme-linked receptor protein signaling pathway,nucleotide excision repair,DNA gap filling.Cytological components were mainly located outside the cell.Molecular functions were enriched in structural molecular activity,receptor binding,serine-type peptidase activity,etc.The analysis of KEGG signaling pathway showed that the differential genes were mainly enriched in the mismatch repair pathway,non-homologous terminal binding pathway and so on.Conclusion:Through the analysis of the differential genes related to the radiosensitivity of prostate cancer,the key differential genes found in this study can help to understand the potential molecular mechanism of prostate cancer radiotherapy resistance,and can provide a theoretical basis for further improving the effectiveness of radiation therapy of prostate cancer.
作者
黄文琳
徐勇
瞿根义
段广军
张玉龙
刘自卫
HUANG Wenlin;XU Yong;QU Genyi;DUAN Guangjun;ZHANG Yulong;LIU Ziwei(The Second Department of Urology,Zhuzhou Hospital Affiliated to Xiangya School of Medicine,Central South University,Hunan Zhuzhou 412007,China)
出处
《现代肿瘤医学》
CAS
北大核心
2022年第6期1056-1059,共4页
Journal of Modern Oncology
基金
湖南省自然科学基金(编号:2020JJ8054)。
