摘要
目的探究大黄素联合康复训练对脑梗死大鼠炎症损伤和神经损伤的改善作用。方法清洁级SD大鼠96只,雌雄各半,鼠龄2~3月,体重200~260 g,由武汉大学动物实验中心提供。按随机数字表分为假手术组、模型组、康复训练组、大黄素+康复训练组,每组各24只。对模型组、康复训练组、大黄素+康复训练组的大鼠采用线栓法制备大脑中动脉栓塞的脑缺血模型,假手术组仅进行颈部血管暴露处理。其中假手术组和模型组进行常规饲养,康复训练组进行转棒训练、网屏训练、跑台训练等训练,大黄素+康复训练组在康复训练组的基础上,联用大黄素进行治疗。在术后第21天,比较各组大鼠神经功能评分;比较细胞色素C(CytC)、Bax、Caspase-3、神经元特异性烯醇化酶(NSE)、S100B等指标含量;比较大鼠缺血侧海马组织中白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)的水平;比较大鼠缺血侧海马组织中NF-κB p65和IκBα蛋白表达水平。结果假手术组大鼠未发现神经功能缺损的症状,经干预后,模型组、康复训练组、大黄素+康复训练组的神经功能评分均随时间延长有所降低,其中模型组的变化最小;与模型组比较,康复训练组、大黄素+康复训练组的神经功能评分明显较低,且大黄素+康复训练组较康复训练组更低,差异均有统计学意义(P<0.05)。与假手术组比较,模型组、康复训练组、大黄素+康复训练组的CytC、Bax、Caspase-3、NSE、S100B、IL-6、IL-1β、TNF-α等指标含量均明显较高,差异均有统计学意义(P<0.05);与模型组比较,康复训练组、大黄素+康复训练组的CytC、Bax、Caspase-3、NSE、S100B、IL-6、IL-1β、TNF-α等指标含量均明显较低,且大黄素+康复训练组较康复训练组更低,差异均有统计学意义(P<0.05)。与假手术组比较,模型组、康复训练组、大黄素+康复训练组的NF-κB p65表达水平明显较高,而IκBα蛋白表达水平明显较低,差异均有统计学意义(P<0.05);与模型组比较,康复训练组、大黄素+康复训练组的NF-κB p65表达水平明显较低,而IκBα蛋白表达水平明显较高,且大黄素+康复训练组较康复训练组变化更显著,差异均有统计学意义(P<0.05)。结论大黄素联合康复训练能够有效降低脑梗死大鼠的神经功能评分,减少脑细胞组织凋亡分子含量,减轻神经损伤程度;其次大黄素联合康复训练能够有效降低脑梗死大鼠炎症因子水平,减轻炎症反应,改善炎症损伤。
Objective To explore the effect of emodin combined with rehabilitation training on inflammatory injury and nerve injury in rats with cerebral infarction.Methods Ninety-six clean-grade SD rats,half female and half male,aged 2-3 months,weighing 200-260 g,were provided by the Animal Experiment Center of Wuhan University,divided into sham operation group,model group,rehabilitation training group,and emodin+rehabilitation training group according to random number table,with 24 rats in each group.The cerebral ischemia model of middle cerebral artery embolism was prepared for rats in the model group,rehabilitation training group,and emodin+rehabilitation training group by suture method,and the sham operation group was only exposed to the neck blood vessels.Among them,the sham operation group and the model group were given routine feeding,the rehabilitation training group was given training such as rotarod training,screen training,and treadmill training,and the emodin+rehabilitation training group was treated with emodin on the basis of the rehabilitation training group.On the 21st day after the operation,the neurological function scores of the rats in each group were compared;the contents of cytochrome C(CytC),Bax,Caspase-3,neuron-specific enolase(NSE),S100B and other indexes were compared;the levels of interleukin(IL)-6,IL-1β,tumor necrosis factor-α(TNF-α)in the hippocampus of the ischemic side of the rats were compared;the protein expression levels of NF-κB p65 and IκBαin the ischemic hippocampus of rats were compared.Results No symptoms of neurological deficit were found in the rats in the sham operation group.After intervention,the neurological function scores of the three groups decreased with time,and the model group had the smallest change.Compared with the model group,the neurological function scores of the rehabilitation training group and the emodin+rehabilitation training group were significantly lower,and the emodin+rehabilitation training group was lower than the rehabilitation training group,and the differences were statistically significant(P<0.05).Compared with the sham operation group,the contents of CytC,Bax,Caspase-3,NSE,S100B,IL-6,IL-1β,TNF-αand other indicators in the model group,rehabilitation training group,and emodin+rehabilitation training group were significantly higher,the differences were statistically significant(P<0.05);compared with the model group,the contents of CytC,Bax,Caspase-3,NSE,S100B,IL-6,IL-1β,TNF-αand other indicators in the rehabilitation training group and the emodin+rehabilitation training group were significantly lower,and the emodin+rehabilitation training group were lower than those in the rehabilitation training group,and the differences were statistically significant(P<0.05).Compared with the sham operation group,the expression levels of NF-κB p65 in the model group,the rehabilitation training group,and the emodin+rehabilitation training group were significantly increased,while the protein expression level of IκBαwas significantly decreased,and the differences were statistically significant(P<0.05).Compared with the model group,the expression level of NF-κB p65 in the rehabilitation training group and the emodin+rehabilitation training group was significantly decreased,while the protein expression level of IκBαwas significantly increased,and the emodin+rehabilitation training group had more significant changes than the rehabilitation training group,and the differences were statistically significant(P<0.05).Conclusion Emodin combined with rehabilitation training can effectively reduce the neurological function score of rats with cerebral infarction,reduce the content of apoptosis molecules in brain cells,and reduce the degree of nerve damage,inflammatory response,improve inflammatory damage.
作者
徐姣
刘扬
崔勇鹤
王震雨
熊小丽
XU Jiao;LIU Yang;CUI Yong-he(Department of Neurology,Xiangyang Central Hospital,Xiangyang Hubei 441000,China;Department of Neurosurgery,Xiangyang Central Hospital,Xiangyang Hubei 441000,China;Department of Critical Medicine,Xiangyang Central Hospital,Xiangyang Hubei 441000,China)
出处
《临床和实验医学杂志》
2022年第4期337-341,共5页
Journal of Clinical and Experimental Medicine
基金
2013-2014、2015-2016年度湖北省卫生计生委科研立项项目(编号:WJ2015Q032)。
关键词
大鼠
脑梗死
大黄素
康复训练
炎症损伤
神经功能损伤
Rats
Cerebral infarction
Emodin
Rehabilitation training
Inflammatory damage
Nerve function damage
