摘要
目的探讨结肠癌患者粪便和癌组织中miR-21和miR-34a甲基化检测及临床意义。方法收集2019年3月至2020年1月在我院进行手术的82例结肠癌患者肿瘤组织标本和癌旁正常结肠黏膜组织样本;同时收集手术前粪便样本,另外收集40例良性结肠肿瘤患者的粪便样本作为对照组。采用实时荧光定量PCR法和甲基化特异性PCR(MSP)法检测样本中miR-21、miR-34a表达和启动子区甲基化状态。另外检测血清肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)的表达量。结果与癌旁组织相比,结肠癌组织中miR-21相对表达量升高,同时miR-34a相对表达量降低(P<0.05);miR-21启动子区甲基化率较癌旁组织降低,miR-34a启动子甲基化率较癌旁组织升高(P<0.05)。与粪便样本对照组相比,结肠癌患者粪便中miR-21相对表达量升高,miR-34a相对表达量降低(P<0.05);miR-21启动子区甲基化率较对照组降低,miR-34a启动子区甲基化率较对照组升高(P<0.05)。miR-21、miR-34a在结肠癌患者肿瘤组织和粪便中的表达量呈正相关性(r=0.782,0.618,P<0.001)。粪便miR-21、miR-34a甲基化检测分别联合粪便隐血均能够提高粪便隐血单独用于结肠癌诊断的敏感性、特异性和检出率(P<0.05)。此外结肠癌患者粪便miR-21和miR-34a甲基化失调与肿瘤直径有关(P<0.05);并且结肠癌患者粪便miR-21非甲基化组血清TNF-α、IL-6水平较甲基化组水平升高,同时粪便miR-34a甲基化组血清TNF-α、IL-6水平较非甲基化组水平亦升高(P<0.05)。结论结肠癌患者粪便和癌组织中miR-21呈低甲基化,同时miR-34a呈高甲基化,联合检测粪便中miR-21、miR-34a甲基化状态可以提高粪便隐血对结肠癌的临床诊断效能。
Objective To investigate the clinical significance of miR-21 and miR-34 a expression and methylation in fecal and tumor tissues of colon cancer patients.Methods From March 2019 to January 2020,82 patients with colon cancer underwent surgery in our hospital were enrolled.Tumor and adjacent colon mucosa samples during operation and stool samples before operation were collected.Stool samples from 40 patients with benign colon tumors were collected as Controls.The expression and methylation status of miR-21 and miR-34 a were detected by real-time PCR and methylation-specific PCR(MSP).Serum tumor necrosis factor α(TNF-α) and interluekin-6(IL-6) were detected.Results Compared with the para-cancer tissue, the expression of miR-21 was increased, while miR-34 a was decreased(P<0.05).Methylation rate of miR-21 promoter in colon cancer was lower, while miR-34 a promoter was higher than that in para-cancer tissue(P<0.05).Compared with Controls, the expression of miR-21 was increased, while miR-34 a was decreased(P<0.05).The methylation rate of miR-21 promoter in colon cancer patients was lower, while miR-34 a promoter was higher than that in control group(P<0.05).Moreover, the expression of miR-21 and miR-34 a in colon cancer tissues and stool was positively correlated(r=0.782,0.618,P<0.001).The sensitivity, specificity and detection rate of colon cancer via fecal occult blood combined with fecal miR-21 and miR-34 a methylation were higher than fecal occult blood alone(P<0.05).miR-21 and miR-34 a methylation disorders in colon cancer patients were correlated with tumor diameter(P<0.05).Serum levels of TNF-α and IL-6 in colon cancer patients with miR-21 non-methylation and miR-34 a methylation were higher(P<0.05).Conclusion miR-21 was demethylationed and miR-34 a was hypermethylated in the fecal and tumor tissues of patients with colon cancer.The combined detection of miR-21 and miR-34 a methylation status in stool can improve the clinical diagnostic efficacy of fecal occult blood for colon cancer.
作者
钟翔
向晓曦
Zhong Xiang;Xiang Xiaoxi(Department of Pathology,Enshi Central Hospital,Enshi Hubei 445000,China;Department of General Surgery,Enshi Central Hospital,Enshi Hubei 445000,China)
出处
《遵义医科大学学报》
2022年第1期92-98,共7页
Journal of Zunyi Medical University
基金
湖北省卫生计生委中医药科研面上项目(NO:ZY2018Z006)。
