摘要
目的使用不同剂量的舒泰联合盐酸赛拉嗪麻醉C57BL/6J小鼠,观察小鼠在不同麻醉剂量下的麻醉相关时间,为各类小鼠手术及相关实验设计提供参考。方法 100只C57BL/6J小鼠(雌雄各半)随机分成5组,每组20只(雌雄各半)。4个给药组小鼠分别按照舒泰55 mg/kg+盐酸赛拉嗪13.75 mg/kg、舒泰65 mg/kg+盐酸赛拉嗪16.25 mg/kg、舒泰75 mg/kg+盐酸赛拉嗪18.75 mg/kg、舒泰85 mg/kg+盐酸赛拉嗪21.25 mg/kg的剂量进行腹腔注射;小鼠翻正反射消失后,于双眼各滴注新乐敦滴眼液1滴(约20μL),放置小鼠于保温垫上。对照组小鼠腹腔注射200μL的0.9%氯化钠溶液。观察、记录并比较各组小鼠的麻醉诱导时间、麻醉维持时间、麻醉恢复时间。小鼠麻醉苏醒后饲养1 d,取血清,采用日立7080全自动生化分析仪测定肝肾功能指标:谷丙转氨酶、谷草转氨酶、肌酐。结果舒泰联合盐酸赛拉嗪麻醉C57BL/6J小鼠的成功率为100%。当舒泰麻醉剂量在55~75 mg/kg时能迅速且安全地诱导小鼠麻醉,麻醉效果良好。麻醉维持时间与舒泰注射剂量成正比(雄鼠r;=0.827,雌鼠r;=0.841,P值均<0.05),麻醉诱导时间与注射剂量成反比(雄鼠r;=0.432,雌鼠r;=0.410,P值均<0.05)。麻醉苏醒后,各组小鼠的肝肾功能指标与对照组相比无明显差异(P>0.05)。结论使用55~75 mg/kg舒泰联合盐酸赛拉嗪麻醉C57BL/6J小鼠可得到较好的麻醉效果且安全,并且可通过调整麻醉药剂量控制麻醉时间,提示这是一种稳定可靠的麻醉方法。
Objective To observe the duration of anesthesia in C57BL/6J mice anesthetized using different doses of Zoletil combined with serazine hydrochloride to provide an effective reference for all types of mouse surgery and related experimental design.Methods One hundred C57BL/6J mice (half male and female) were randomly divided into five groups,with 20 mice (half male and female) in each group.The mice in the four groups were intraperitoneally injected with 55 mg/kg Zoletil and 13.75 mg/kg serazine hydrochloride,65 mg/kg Zoletil and 16.25 mg/kg serazine hydrochloride,75 mg/kg Zoletil and 18.75 mg/kg serazine hydrochloride,or 85 mg/kg Zoletil and 21.25 mg/kg serazine hydrochloride,respectively.After the righting reflex stopped,one drop (about 20μL) was injected into each eye,and the mice were placed on a heat preservation pad.Mice in the control group were intraperitoneally injected with 200μL normal saline.Differences in the anesthesia induction time,maintenance time,and awakening time were observed and compared.The mice were fed for one day after anesthesia;serum was collected,and liver and kidney function indexes such as alanine aminotransferase (ALT),aspartate aminotransferase (AST),and creatinine(CREA) were determined using Hitachi 7080 automatic biochemical analyzer.Results The success rate of anesthesia in C57BL/6J mice treated with Zoletil combined with serazine hydrochloride was 100%.When the anesthetic dose was 55-75 mg/kg,the anesthesia was induced quickly and safely,and the anesthetic effect was good.The duration of anesthesia was proportional to the injection dose of Zoletil (r;=0.827 in male mice,r;=0.841 in female mice,both P<0.01),and the induction time was inversely proportional to the injection dose (r;=0.432 in male mice,r;=0.410 in female mice,both P<0.05).However,there was no significant difference in liver and kidney function between the groups and the control group (P>0.05).Conclusion Anesthetizing C57BL/6J mice with Zoletil combined with serazine hydrochloride is a reliable and stable method,and the duration of anesthesia can be controlled by adjusting the dosage.
作者
王成稷
柴青
龚慧
王珏
万颖寒
顾正页
暴旭
沈如凌
WANG Chengji;CHAI Qing;GONG Hui;WANG Jue;WAN Yinghan;GU Zhengye;BAO Xu;SHEN Ruling(Shanghai Laboratory Animal Research Center,Shanghai 201203,China;Qingdao Municipal Center for Disease Prevention and Control,Qingdao 266011,China;Suzhou Kunchen Biotechnology Co.,Ltd.,Suzhou 200231,China)
出处
《实验动物与比较医学》
CAS
2022年第1期31-35,共5页
Laboratory Animal and Comparative Medicine
关键词
舒泰
盐酸赛拉嗪
麻醉
小鼠
Zoletil
Serazine hydrochloride
Anesthesia
Mice
