摘要
采用闪式法从沙棘叶中提取总黄酮,利用正交试验优化提取工艺条件,并与传统浸提法进行比较。结果表明,闪式法提取沙棘叶总黄酮的最佳工艺为提取电压180 V、提取时间120 s、乙醇体积分数60%、液料比(V_(乙醇)︰m_(沙棘叶)) 18︰1 mL/g。HPLC分析发现粗提液中含有杨梅素、槲皮素、山奈酚和异鼠李素。闪式提取法与传统浸提法提取的沙棘叶总黄酮的得率和粗提物组分基本无差异,但闪式提取短时、低温,能有效降低提取成本。
Total flavonoids were extracted from seabuckthorn leaves by flash extraction method.The extraction conditions were optimized by orthogonal test.The flash extraction of total flavonoids was compared with that of traditional extraction method.The results showed that the optimal flash extraction conditions were extraction voltage 180 V,extraction time 120 s,ethanol volume percentage 60% and liquid-solid ratio(V_(ethanol)︰m_(seabuckthorn leaves)) 18︰1 mL/g.HPLC analysis showed that the flash crude extract contained myricetin,quercetin,kaempferol and isorhamnetin.The flavonoids extraction yield and crude extract flavonoids components of the flash method from seabuckthorn leaves were almost consistent with that of traditional extraction method.However,the short-term and low temperature of flash extraction could effectively reduce the extraction cost.
作者
张存存
张娟
谭志超
柳嘉
王永霞
ZHANG Cuncun;ZHANG Juan;TAN Zhichao;LIU Jia;WANG Yongxia(Zhangbei Baodekang Food Co.,Ltd.,Zhangjiakou 076450;China National Research Institute of Food&Fermentation Industries Co.,Ltd.,Beijing 100015;Hebei University of Engineering,Handan 056038)
出处
《食品工业》
CAS
2022年第1期1-5,共5页
The Food Industry
基金
河北省科学技术厅重点研发计划项目(编号:19223002D)。
关键词
沙棘
总黄酮
闪式提取
组分鉴定
高效液相色谱
seabuckthorn
total flavonoids
flash extraction
component identification
high performance liquid chromatography
