摘要
为明确莲藕采后主要致腐真菌的种类,本研究采用组织分离法对在25℃贮藏条件下自然腐烂的莲藕进行病原菌分离纯化及致病性检测,对主要致腐真菌进行形态学及分子生物学鉴定,并研究其生物学特性。结果表明,从常温下自然腐烂的莲藕中分离出LO-1、LO-2、LO-3共3种真菌;回接试验结果表明,LO-1是主要致腐菌,经鉴定为可可毛色二孢菌(Lasiodiplodia theobromae)。菌株的生物学特性研究结果表明,该菌最适培养温度为25℃,持续光照可促进其生长;55℃处理10 min可致死;在pH值4~10条件下均能生长,在pH值为5时扩展直径最大;以蔗糖为碳源、酵母浸出粉为氮源时最利于菌丝生长;在莲藕汁-葡萄糖培养基上菌丝扩展直径最大,持续光照可促进孢子产生。莲藕采后主要致腐真菌的确定为后续采取针对性的防腐保鲜措施提供了依据,为常温下莲藕采后贮藏提供理论基础。
To elucidate the dominant pathogenic fungi of postharvest lotus roots,the pathogens were isolated and purified from the naturally rotten samples stored at 25℃.The dominant pathogenic strains were identified by morphological and molecular biology assay,and their biological characteristics were investigated.Three fungi named LO-1,LO-2,LO-3 were isolated from the naturally decomposed lotus roots,and LO-1,identified as Lasiodiplodia theobromae,was the dominant pathogenic fungus by pathogenicity tests.The optimal temperature for mycelial growth of L.theobromae was 25℃and continuous light could promote the mycelium growth,while the lethal temperature was 55℃for 10 min.The strain could grow at pH 4~10 and the largest expansion diameter was at pH 5.The optimal carbon and nitrogen source for L.theobromae growth was sucrose and yeast extract.The largest expansion diameter of mycelia was measured on lotus root juice-glucose media.The oatmeal media and continuous light could promote the spore production of L.theobromae.This study would set up a targeted technical guidance for the preservation and prolongation of the storage time of lotus roots.
作者
贾乐
刘瑞玲
房祥军
陈杭君
郜海燕
JIA Le;LIU Ruiling;FANG Xiangjun;CHEN Hangjun;GAO Haiyan(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,Zhejiang 321000;Food Science Institute,Zhejiang Academy of Agricultural Science/Key Laboratory of Post-Harvest Handling of Fruits,Ministry of Agriculture and Rural Affairs/Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province,Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables,China National Light Industry,Hangzhou,Zhejiang 310021)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第3期661-668,共8页
Journal of Nuclear Agricultural Sciences
基金
现代农业产业技术体系专项资金(CARS-24-E-01)
浙江省万人计划人才培养项目(2018R52020)
浙江省重点研发计划项目(2019C02079)。
关键词
莲藕
致腐真菌
分离鉴定
生物学特性
lotus root
spoilage fungus
isolation and identification
biological characteristics
