摘要
目的:探讨中波紫外线(UVB)辐射导致人角质形成细胞(HaCaT细胞)光老化的模型构建及机制研究。方法:体外培养HaCaT细胞分为空白组和实验组,模型组细胞用不同照射剂量的UVB照射,空白组细胞不予紫外线照射,采用CCK-8法检测细胞相对活力,筛选出最适合的照射剂量用于建立光老化模型;应用同样的方法体外培养HaCaT细胞,分为空白组和模型组,模型组细胞使用筛选出的UVB照射剂量,空白组细胞不予紫外线照射,应用试剂盒检测光老化细胞模型的相关因子。结果:照射剂量为60 mj/cm2时,细胞增殖率接近空白组的50%(P<0.05),最适合建立光老化模型;与空白组比较,模型组细胞中活性氧(ROS)、丙二醛(MDA)含量均升高(均P<0.05),超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)含量及总抗氧化能力(T-AOC)水平均降低(均P<0.05);白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)的含量均升高(均P<0.05)。结论:UVB照射剂量60 mj/cm2最适合用于建立光老化人角质形成细胞模型,可能是UVB照射导致HaCaT细胞光老化与细胞内的抗氧化水平降低、炎症因子含量升高有关。
Objective:To investigate the photoaging model and mechanism of human keratinocytes(HaCaT cells)induced by UVB radiation.Methods:HaCaT cells were cultured in vitro and divided into blank group and model group.Cells in the model group were irradiated with different doses of UVB,while cells in the blank group didn’t receive irradiation.The relative activity of cells was detected by CCK-8 method,and the most suitable irradiation dose was selected to establish the photoaging model.HaCaT cells were cultured in vitro using the same method,and were divided into blank group and model group.Cells in the model group were irradiated with screened UVB radiation dose,while cells in the blank group didn’t receive irradiation.Results:The radiation dose of 60 mj/cm^(2) was the most suitable for the establishment of photoaging model,with cell proliferation rate nearly 50%of that in the blank group(P<0.05).Compared with blank group,reactive oxygen species(ROS)and malonaldehyde(MDA)contents in model group were significantly increased(P<0.05),superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx)contents and total antioxidant capacity(T-AOC)levels were significantly decreased(P<0.05);The contents of Interleukin-1(IL-1),Interleukin-6(IL-6)and tumor necrosis factor(TNF-α)were significantly increased(P<0.05).Conclusion:UVB irradiation dose of 60mj/cm^(2) is the most suitable for the establishment of photoaged human keratinocyte model.The photoaging of HaCaT cells induced by UVB irradiation is related to the decrease of intracellular antioxidant level and the increase of inflammatory factors.
作者
罗秀玲
周源
赵斌斌
黄曼丽
李季超
李文宇
范润哥
温斯健
林有坤
Luo Xiuling;Zhou Yuan;Zhao Binbin;Huang Manli;Li Jichao;Li Wenyu;Fan Runge;Wen Sijian;Lin Youkun(Department of Dermatology,Wuming Hospital of Guangxi Medical University,Nanning 530100,China;Department of Dermatology,The People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530022,China;Guangxi Helanmo Technology Co,LTD,Nanning 530000,China;Department of Dermatology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2022年第2期246-250,共5页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81760561)
广西自然科学基金资助项目(No.2020GXNSFAA297150)。
