摘要
本研究建立了茶叶中高氯酸盐的离子色谱串联质谱分析方法.对样品前处理方法进行了优化,结果表明用20 mL高纯水振荡提取15 min,重复3次,第3次高氯酸盐浸出量仅为总浸出量的2.3%—3.0%,因此可认为茶叶经3次浸提后高氯酸盐浸出接近完全.样品萃取液选用AS20色谱柱进行分离和电喷雾串联质谱(ESI-MS/MS)检测,样品在加标100、300、600μg·kg^(-1)的情况下,高氯酸盐回收率分别在94.6%—133%、101%—118%和101%—109%之间.方法在0.05—50μg·L^(-1)之间具有良好的线性关系,定量限为6μg·kg;.最后将方法用于不同类茶叶中高氯酸盐的分析,均检出不同浓度高氯酸盐的存在,浓度在97.0—2.71×10^(3)μg·kg^(-1)之间,通过茶叶摄入高氯酸盐对人体造成的暴露需引起人们的关注.
A method was established for the determination of perchlorate in tea using ion chromatography coupled with tandem mass spectrometry.The result showed that the amount of perchlorate after the third extraction process accounts only 2.3%—3.0%of the total amount of perchlorate by shaking extraction with 20 mL high purity water for 15 min and repeating for three times.Therefore,the perchlorate in tea was considered to be completely extracted after three times extraction.After extraction,the sample was separated using an AS20 column with the detection using electrospray tandem mass spectrometry(ESI-MS/MS).The recoveries of perchlorate were within the ranges of 94.6%—133%,101%—118%and 101%—109%when the sample was spiked with the concentrations of 100,300 and 600μg·kg^(-1),respectively.The method showed a good linear range between 0.05—50μg·L^(-1) with the limit of quantification at 6μg·kg^(-1).Finally,the proposed method was successfully applied to determine perchlorate in different types of tea.The result showed that perchlorate could be detected in all samples with the concentrations between 97.0μg·kg^(-1) and2.71×10^(3)μg·kg^(-1).The human exposure risks of perchlorate through tea intake needs to be concerned.
作者
张志敏
史亚利
王文倩
孙桂容
张春晖
蔡亚岐
ZHANG Zhimin;SHI Yali;WANG Wenqian;SUN Guirong;ZHANG Chunhui;CAI Yaqi(School of Chemical&Environmental Engineering,China University of Mining&Technology(Beijing),Beijing,100083,China;State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,100085,China)
出处
《环境化学》
CAS
CSCD
北大核心
2022年第2期572-580,共9页
Environmental Chemistry
基金
国家自然科学基金(21722705)资助。
关键词
高氯酸盐
前处理过程
离子色谱串联质谱
茶叶
perchlorate
pretreatment
ion chromatography tandem mass spectrometry
tea
