摘要
目的分析不同耐药鲍曼不动杆菌外排泵基因及群体信号系统基因的分布,探讨不同耐药基因型鲍曼不动杆菌与抗菌药物耐药的特点。方法收集2018年1月至2021年7月皖南医学院第二附属医院130株鲍曼不动杆菌,按耐药性不同分为多重耐药菌31株、碳青霉烯类耐药菌43株和非多重耐药菌56株,分析各组鲍曼不动杆菌对抗生素的耐药性,采用PCR检测以上3组菌株外排泵基因ade B、ade J、abe S、abe M和群体信号系统基因Aba I、Aba R分布情况。结果多重耐药和碳青霉烯类耐药菌株对头孢他啶、头孢曲松、环丙沙星、左氧氟沙星、复方新诺明、哌拉西林/他唑巴坦耐药率均大于60%。130株鲍曼不动杆菌中,基因检测结果显示ade B、ade J、abe S、abe M、Aba I、Aba R检出率分别是43.85%、48.46%、46.15%、48.46%、46.15%、48.46%。其中非多重耐药菌株组携带外排泵基因及群体信号系统基因均低于其余两组,群体信号系统基因Aba I、Aba R在碳青霉烯类耐药组中检出率较多重耐药组增高,差异具有统计学意义(χ^(2)=6.049、6.188,P均<0.05)。结论临床分离的鲍曼不动杆菌耐药性广泛,鲍曼不动杆菌对碳青霉烯类药物的耐药性与携带群体信号系统基因Aba I、Aba R密切相关。
Objective To analyze the distribution of different drug-resistant Acineto-bacter baumannii with efflux pump genes and Quorum sensing system genes,and explore the characteristics of different drug resistant genotypes of Acinetobacter baumannii and antimicrobial resistance.Methods From January 2018 to July 2021,130 strains of Acinetobacter baumannii from the Second Affiliated Hospital of Wannan Medical College were collected and divided into multidrug-resistant strains(31 strains),carbapenem-resistant strains(43 strains)and nondrug-resistant strains(56 strains)according to their drug resistance.The antibiotic resistance of Acinetobacter baumannii in each groupwas analysed.The efflux pump genes ade B,ade J,abe S and abe M,and Quorum sensing system genesAba I,Aba Rwere detected by PCR.Results The resistance rates of multidrug-resistant and carbapenem-resistant strains to ceftazidime,ceftriaxone,ciprofloxacin,levofloxacin,co-trimoxazole,piperacillin/tazobactam were all>60%among the 130 strains of Acinetobacter baumannii.The results showed that the detection rateof ade B,ade J,abe S,abe M,Aba I,and Aba R was 43.85%,48.46%,46.15%,48.46%,46.15%,48.46%,respectively.Among them,the efflux pump genes and Quorum sensing system genes in the non multidrug resistant strain group were lower than the other two groups.The detection rates of Quorum sensing system genes Aba I and Aba R in the carbapenem-resistant group were significantly higher than thosein multidrug resistant group(x 2=6.049,6.188;both P<0.05).Conclusions The clinically isolated Acinetobacter baumannii has serious drug resistance,and the carbapenem-resistance is closely related to carrying Quorum sensing system genes.
作者
帅朝霞
钟峰
许岩
吴竞
SHUAI Zhaoxia;ZHONG Feng;XU Yan;WU Jing(Department of Clinal Laboratory,the Second Affiliated Hospital of Wannan Medical College,Wuhu 241000,China)
出处
《安徽医学》
2022年第3期275-279,共5页
Anhui Medical Journal
基金
国家自然科学基金(项目编号:81601806)。
关键词
鲍曼不动杆菌
多重耐药菌
外排泵机制
群体信号系统
Acinetobacter baumannii
Multi-drug resistant bacteria
Efflux pump mechanism
Quorum sensing system
