摘要
目的考察毛萼乙素(Eriocalyxin)是否通过调控长链非编码RNA(lncRNA)CPS1 intronic transcript 1(CPS1-IT1)的表达影响膀胱癌T24细胞的增殖、迁移、侵袭。方法四甲基偶氮唑蓝(MTT)测定法检测(2.5μmol/L、5μmol/L、10μmol/L)毛萼乙素作用于T24细胞的增殖,免疫印迹法(Western blot)测定Ki-67、基质金属蛋白酶(MMP)-2和MMP-9蛋白表达,Transwell法评估T24细胞的迁移、侵袭;实时荧光定量PCR(qRT-PCR)分析lncRNA CPS1-IT1表达。T24细胞转染pcDNA-CPS1-IT1、或转染si-CPS1-IT1并给予10μmol/L毛萼乙素,考察T24细胞的增殖、迁移、侵袭变化。结果(2.5μmol/L、5μmol/L、10μmol/L)毛萼乙素增加T24细胞的增殖抑制率和lncRNA CPS1-IT1表达量,减少迁移、侵袭细胞数、Ki-67、MMP-2、MMP-9蛋白的表达量,呈一定的浓度依赖性,差异均有统计学意义(P<0.05)。lncRNA CPS1-IT1过表达提高T24细胞的抑制率,降低迁移、侵袭细胞数、Ki-67、MMP-2和MMP-9蛋白表达量,差异均有统计学意义(P<0.05)。抑制lncRNA CPS1-IT1表达逆转了毛萼乙素(10μmol/L)对T24细胞增殖、迁移、侵袭的影响。结论毛萼乙素通过上调lncRNA CPS1-IT1表达,抑制膀胱癌T24细胞的增殖、迁移、侵袭。
Objective To investigate whether eriocalyxin B(ERB)affects the proliferation,migration and invasion of bladder cancer T24 cells by regulating the expression of long non-coding RNA(lncRNA)CPS1-IT1.Methods Thiazole blue(MTT)assay was used to detect the proliferation of T24 cells treated by eriocalyxin B(2.5μmol/L,5μmol/L,10μmol/L).Western Blot was used to determine the expression levels of Ki-67 and matrix metalloproteases(MMP)-2 as well as MMP-9 protein.Transwell was used to evaluate the migration and invasion of T24 cells,and real-time fluorescent quantitative PCR(qRT-PCR)was used to analyze the expression of lncRNA CPS1-IT1.Moreover T24 cells were transfected with pcDNA-CPS1-IT1,or transfected with si-CPS1-IT1 and were treated by 10μmol/L eriocalyxin B to investigate the proliferation,migration,and invasion of T24 cells.Results Eriocalyxin B(2.5μmol/L,5μmol/L,10μmol/L)significantly increased the proliferation inhibition rate of T24 cells and the expression levels of lncRNA CPS1-IT1,and decreased the number of migration and invasion cells,and the expression levels of Ki-67,MMP-2 MMP-9 protein,with a certain concentration-dependent manner(P<0.05).The overexpression of lncRNA CPS1-IT1 significantly improved the inhibition rate of T24 cells,decreased the number of migration and invasion cells,and the expressionlevels of Ki-67,MMP-2 and MMP-9 proteins(P<0.05).Inhibiting the expression of lncRNA CPS1-IT1 reversed the effects of eriocalyxin B(10μmol/L)on the proliferation,migration and invasion of T24 cells.Conclusion Eriocalyxin B can inhibit the proliferation,migration and invasion of bladder cancer T24 cells by up-regulating the expression of lncRNA CPS1-IT1.
作者
熊涛
王慧
黄梦婷
陈铮锐
XIONG Tao;WANG Hui;HUANG Mengting(Department of Urology,Rongchang District People’s Hospital,Chongqing 402460,China;不详)
出处
《河北医药》
CAS
2022年第5期650-654,共5页
Hebei Medical Journal
基金
重庆市医学科研项目(编号:2015MSXM186)。
