摘要
为明确不同有机物浓度(50~150mg/L)和竹炭同时存在下厌氧氨氧化颗粒污泥系统的脱氮除碳功能菌群结构及代谢途径差异,采用宏基因组测序技术对其微生物分布规律和碳氮代谢基因表达进行了研究.结果表明,当COD浓度为50,150mg/L,添加竹炭显著提升了厌氧氨氧化菌(AnAOB)的相对丰度,Candidatus_Kuenenia的相对丰度分别由2.0%和2.9%增加至1.8%和4.5%.与只添加有机物的对照组相比,添加竹炭的处理组显著改变了微生物群落结构,在炭存在下异化硝酸盐还原为铵(DNRA)细菌丰度下降,反硝化菌属和碳代谢相关菌属丰度增加,说明竹炭的投加有助于维持厌氧氨氧化、反硝化和DNRA3个氮代谢途径群落结构的稳定.微生物共现网络分析表明,不同脱氮菌群的协同作用提高了总氮(TN)的去除率,竹炭可以通过Halomonas的富集提高Candidatus_Brocadia和Candidatus_Jettenia的抗逆性.通过KEGG数据库注释表明,有机物存在条件下竹炭提升了厌氧氨氧化颗粒污泥系统的碳氮代谢效能,尤其促进了糖酵解途径(EMP)与三羧酸循环(TCA cycle)的衔接.
In order to clarify the differences of the nitrogen and carbon removal functional bacteria community structure and metabolic pathway in the anammox granular sludge system with different concentrations of organic matter(50~150 mg/L) and bamboo biochar, the microbial distribution and gene expression of carbon and nitrogen metabolism were studied by metagenomic sequencing in this paper. The results showed that the bamboo charcoal addition was significantly increased the relative abundance of anammox when the COD concentration was 50 and 150 mg/L, the relative abundance of Candidatus_Kuenenia increased from 2.0%and 1.8% to 2.9% and 4.5%. In addition, compared with the control group only with organic matter(without bamboo biochar), the bamboo biochar addition was significantly changed the microbial community structure. The abundance of DNRA(dissimilatory nitrate reduction to ammonium process) bacteria was decreased, while the abundance of denitrifying bacteria and carbon metabolism-related bacteria were increased, which indicated that bamboo biochar addition was helpful to maintain the stability of nitrogen metabolism pathway community structure of anammox, denitrification and DNRA. The network analysis of microorganisms was showed that the removal efficiency of TN was increased by the synergetic effect of different nitrogen removal bacteria groups.However, the resistance of Candidatus_Brocadia and Candidatus_Jettenia could be improved by Halomonas enrichment with bamboo biochar addition. The KEGG annotation indicated that bamboo charcoal addition would be enhanced the carbon and nitrogen metabolism efficiency of anammox granular sludge system in the presence of organic matter, especially promoted the connection between EMP(glycolytic pathway) and TCA(tricarboxylic acid cycle).
作者
常尧枫
郭萌蕾
谢军祥
谢嘉玮
陈重军
CHANG Yao-feng;GUO Meng-lei;XIE Jun-xiang;XIE Jia-wei;CHEN Chong-jun(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Key Laboratory of Environmental Science and Engineering,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou 215009,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2022年第3期1138-1145,共8页
China Environmental Science
基金
江苏省自然科学基金资助项目(BK20201450)
中国博士后科学基金资助项目(2020M671400)
苏州市民生科技项目(SS202016,SS2019022)
江苏水处理技术与材料协同创新中心预研项目(XTCXSZ2019-3)。
关键词
宏基因组
厌氧氨氧化颗粒污泥
脱氮除碳
菌群结构
代谢途径
metagenomics
anammox granular sludge
nitrogen and carbon removal
bacteria community structure
metabolism pathway
