摘要
对食用油中苯并(a)芘分子印迹固相萃取法测定过程中的试剂及耗材进行污染溯源分析。结果表明:分析纯试剂及一次性注射器中的橡胶是污染的主要来源,二氯甲烷(分析纯)、正己烷(分析纯)、橡胶的苯并(a)芘平均峰面积分别为0.261、0.710、2.550 mV·min,分别是国标方法检出限的0.9、2.4、8.7倍,对检测结果准确性带来严重影响。因此,提出使用高速离心替代过滤膜方式除杂以除去样液中微小颗粒物,该方案既能避免注射器带来的污染,又能保证高效液相色谱系统稳定运行,提高检验结果准确性。
The pollution traceability analysis of reagents and consumables used in determination of benzo(a)pyrene(BaP) in edible oil by molecularly imprinted solid phase extraction was conducted. The results showed that analytical reagent and rubber in syringe were the main sources of BaP pollution. The average peak areas of dichloromethane(analytical reagent), n-hexane(analytical reagent) and rubber were 0.261, 0.710 mV·min and 2.550 mV·min, respectively, which were 0.9, 2.4 times and 8.7 times of the detection limit of national standard method, bringing serious impact on the test result accuracy. Therefore, it was proposed to use high-speed centrifugation instead of filtration membrane to remove impurities in sample solution, which not only avoided the pollution caused by the syringe, but also ensured the stable operation of the liquid chromatography system to improve the accuracy of the test results.
作者
郑耀林
张树权
林秋凤
吴佳锋
吴斯敏
莫淑梅
ZHENG Yaolin;ZHANG Shuquan;LIN Qiufeng;WU Jiafeng;WU Simin;MO Shumei(Dongguan Institute for Food and Drug Control,Dongguan 523808,Guangdong,China;School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510640,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第12期84-87,共4页
China Oils and Fats
基金
东莞市社会科技发展一般项目(2020507101631)。
关键词
苯并(A)芘
污染溯源
食用油
分子印迹固相萃取法
benzo(a)pyrene
pollution traceability
edible oil
molecularly imprinted solid phase extraction
