摘要
为探究Frankia菌—细枝木麻黄(Casuarina cunninghamiana)共生体对土壤Pb污染的响应,采用盆栽试验,本试验研究了一定铅(Pb)处理下施用Frankia湿菌体0(CK)、10、20 mg·株^(−1)对细枝木麻黄结瘤量、地上部分Pb、K、Ca、Mg、Cu、Mn的含量、逆境生理以及根系活力的影响;经本课题组预实验结果表明,细枝木麻黄600 mg·kg^(−1)铅(Pb)处理下受到胁迫但不致死,因此正式试验中Pb浓度设为600 mg·kg^(−1)。结果表明,施用Frankia菌湿菌体20 mg·株^(−1)可显著提高细枝木麻黄结瘤量,接种10、20 mg·株^(−1)时,细枝木麻黄结瘤率均为100%。与CK相比,施用Frankia湿菌体10、20 mg·株^(−1),细枝木麻黄地上部Pb含量显著下降;接种量为10 mg·株^(−1)时,细枝木麻黄地上部K含量显著下降,Cu含量显著升高;Ca、Mg、Mn元素变化不显著(P<0.05)。Frankia接种量为20 mg·株^(−1)时,细枝木麻黄体内游离脯氨酸和可溶性糖相比对照组分别下降了56.04%和10.10%,根系活力TTC还原强度上升了87.12%。综上所述:在同一Pb胁迫下,高接种量(20 mg·株^(−1))Frankia菌可以增强细枝木麻黄对土壤Pb污染的抵御能力。
In order to explore the response of Frankia bacteria and Casuarina cunninghamiana symbiont to soil Pb pollution in soil,pot experiments were conducted to study the effects of Frankia wet bacteria 0(CK),10,20 mg·plant^(−1) on nodulation amount,contents of Pb,K,CA,Mg,Cu,Mn in the aboveground parts,stress physiology and root activity of Casuarina cunninghamiana under certain Pb treatment.The results of the pre-experiment showed that Casuarina cunninghamiana was stressed but not lethal under 600 mg·kg^(−1) lead(PB),so the Pb concentration was set at 600 mg·kg^(−1) in the formal experiment.The results showed that the application of 20 mg·plant^(−1) Frankia wet mycelium could significantly increase the nodulation rate of Casuarina cunninghamiana.The nodulation rate of Casuarina cunninghamiana was 100%when inoculated with 10 mg·plant^(−1) or 20 mg·plant^(−1) Frankia wet mycelium.Compared with CK,the Pb content in aerial parts of Casuarina cunninghamiana decreased significantly with the application of Frankia wet mycelium 10 and 20 mg·plant^(−1).When the inoculation amount was 10 mg·plant^(−1),the K content in aerial parts of Casuarina cunninghamiana decreased significantly,while the Cu content increased significantly.The change of Ca,Mg and Mn was not significant(P<0.05).The free proline and soluble sugar in Casuarina cunninghamiana decreased by 56.04%and 10.10%respectively,and the TTC reduction intensity of root activity increased by 87.12%when Frankia inoculation was 20 mg·plant^(−1).In conclusion,under the same Pb stress,high inoculation amount(20 mg·plant^(−1))of Frankia bacteria could enhance the resistance of Casuarina cunninghamiana to Pb pollution in soil.
作者
赵佳怡
熊茂岑
王鑫
钟镆宇
王婷
潘远智
ZHAO Jiayi;XIONG Maocen;WANG Xin;ZHONG Moyu;WANG Ting;PAN Yuanzhi(College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川林业科技》
2022年第1期31-36,共6页
Journal of Sichuan Forestry Science and Technology
基金
大学生创新训练计划项目(201810626010)
国家自然科学基金项目(31670622)。
