摘要
目的 探讨MALAT1靶向miR-204/线粒体转录因子A (TFAM)轴对皮肤鳞状细胞癌恶性生物学行为的影响。方方法 lncRNA芯片筛选皮肤鳞状细胞癌中差异表达的lncRNA;qRT-PCR检测用于分析MALAT1与miR-204的表达;免疫组织化学法与Western blotting检测TFAM的表达;si-阴性对照、si-MALAT1、miR-204类似物、类似物阴性对照、miR-204抑制剂、抑制剂阴性对照、pcDNA3.1质粒与pcDNA3.1-TFAM质粒分别转染皮肤鳞状细胞癌SCL-1细胞;荧光素酶活性分析实验用于检测MALAT1与miR-21,TFAM与miR-21的靶向关系;细胞克隆形成实验及流式细胞术用于检测SCL-1细胞的增殖及凋亡。结果 MALAT1与TFAM在皮肤鳞状细胞癌组织及细胞中高表达,而miR-204在皮肤鳞状细胞癌组织及细胞中低表达。沉默MALAT1或过表达miR-204,均可抑制TFAM蛋白表达,抑制SCL-1细胞增殖,促进细胞凋亡。结论 MALAT1靶向miR-204/TFAM轴影响皮肤癌细胞增殖及凋亡。
Objective To investigate the effect of MALAT1 targeting miR-204/TFAM axis on proliferation and apoptosis of skin squamous cell carcinoma. Method lncRNA array was used to detect the differential expression of lncRNA in skin squamous cell carcinoma;qRT-PCR detection was performed to analyze the expression of MALAT1 and miR-204;Immunohistochemistry and Western blotting were used to detect the expression of TFAM;si-NC, si-MALAT1, miR-204 mimic, mimic NC, miR-204 inhibitor, inhibitor NC, pcDNA3.1 plasmid and pcDNA3.1-TFAM plasmid were respectively transfected into skin squamous cell carcinoma SCL-1 cell;Luciferase activity analysis experiment was used to detect the targeting relationship between MALAT1 and miR-21, TFAM and miR-21;Cell clone formation experiment and flow cytometry were evaluated to detect the proliferation and apoptosis of SCL-1 cells. Results MALAT1 and TFAM were highly expressed in skin squamous cell carcinoma tissue and cells, while miR-204 was low expressed in skin squamous cell carcinoma tissue and cells;silencing MALAT1 or overexpression of miR-204, inhibited TFAM protein expression, inhibited SCL-1 cell proliferation, and promoted apoptosis. Conclusion MALAT1 regulated the cell proliferation and apoptosis by targeting the miR-204/TFAM axis in skin squamous cells.
作者
范荣辉
徐子寒
慕生枝
李罡
Fan Rong-hui;Xu Zi-han;Mu Sheng-zhi;Li Gang(Burn and Plastic Surgery,Shaanxi Provincial People's Hospital,Xi'an 710032,China;School of Basic Medicine,Xi'an Jiaotong University,Xi'an 710061,China)
出处
《兰州大学学报（医学版）》
2022年第2期9-15,共7页
Journal of Lanzhou University（Medical Sciences）
