摘要
为了解秦皇岛地区鸡场沙门菌的感染及耐药情况,试验采集肛拭子样品252份,采用细菌分离培养、生化试验与PCR方法进行细菌鉴定,通过血清凝集试验确定其血清型,利用药敏纸片法测定所分离的沙门菌菌株对21种抗生素的药物敏感性,通过PCR方法检测所分离的沙门菌的耐药基因。结果表明:分离出沙门菌43株,分离率为17.06%。鉴定出5种血清型,以肠炎沙门菌(17株,占39.53%)和鸡白痢沙门菌(13株,占30.23%)为优势血清型。分离得到的沙门菌对青霉素、磺胺异口恶唑等4种抗生素的耐药率在90%以上;对环丙沙星、复方新诺明、庆大霉素等7种抗生素的耐药率为72.09%~88.37%;对氟苯尼考、多黏菌素B、恩诺沙星等7种抗生素的耐药率为51.16%~67.44%;对大观霉素和头孢曲松的耐药率分别为23.26%、27.91%;对氧氟沙星的耐药率最低,为4.65%。且多重耐药现象严重,最高对20种抗生素耐药。检测出18种耐药基因,blaTEM、aadA1、sul2耐药基因的检出率最高,均为97.67%;blaOXA、aadA2、aacC4和aac(6)-Ib耐药基因的检出率均为90.70%;catA1、sul1、tetB耐药基因的检出率为69.77%~88.37%;aacC2、qnrA、qnrB、sul3、floR、aadB、tetA和qnrS耐药基因的检出率为4.65%~44.19%;blaPSE、blaSHV、blaCMY、tetG和cmlA未检出。说明秦皇岛地区鸡源沙门菌病较为流行,且耐药现象较为严重。
In order to understand the infection and drug resistance of Salmonella in Qinhuangdao area, 252 samples of anal swabs were collected in this experiment;bacterial isolation culture, biochemical test and PCR method were used for bacterial identification. The serotypes were determined by serum agglutination test;the susceptibility of the isolated Salmonella strains to 21 antibiotics was determined by the drug sensitive disc method;resistance genes of isolated Salmonella were detected by PCR method. The results showed that 43 strains of Salmonella were isolated, and the separation rate was 17.06%. Five serotypes were identified, with Salmonella enterica subsp. enterica serovar Enteritidis(17, 39.53%) and Salmonella enterica subsp. enterica serovar Pullorum(13, 30.23%) as the dominant serotypes. The isolated Salmonella bacteria had resistance rates of over 90% to four antibiotics, including penicillin and sulfisoxazole;the strains had resistance rates of 72.09%-88.37% to 7 antibiotics, including ciprofloxacin, compound trimethoprim and gentamicin;the strains had resistance rates of 51.16%-67.44% to 7 antibiotics such as florfenicol and polymyxin B;the strains had resistance rates of 23.26% and 27.91% to spectinomycin and ceftriaxone, respectively;the strains had a lowest resistance rate of 4.65% to ofloxacin. And the phenomenon of multi-drug resistance was serious, with the highest resistance to 20 kinds of antibiotics. 18 drug resistance genes were detected. The detection rates of blaTEM, aadA1, sul2 drug resistance genes were 97.67%, which were the highest;the detection rates of blaOXA, aadA2, aacC4 and aac(6)-Ib drug resistance genes were all 90.70%;the detection rates of catA1, sul1, tetB resistance genes were 69.77%-88.37%;the detection rates of aacC2, qnrA, qnrB, sul3, floR, aadB, tetA and qnrS resistance genes were 4.65%-44.19%;the detection results of blaPSE, blaSHV, blaCMY, tetG and cmlA were negative. The results suggested that chicken-derived salmonellosis was prevalent in Qinhuangdao area, and drug resistance phenomenon was serious.
作者
杨瑞
何云凤
刘梦雪
陈玥
党儒尧
李蕴玉
李佩国
YANG Rui;HE Yunfeng;LIU Mengxue;CHEN Yue;DANG Ruyao;LI Yunyu;LI Peiguo(Hebei Province Key Laboratory of Preventive Veterinary Medicine,Hebei Normal University of Science and Technology,Qinhuangdao 066604,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第4期64-68,136,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(HBCT2013090203)
河北省农业农村厅2021科技项目(冀农科21021)。
关键词
鸡
沙门菌
血清型
抗生素
耐药性
耐药基因
chicken
Salmonella
serotype
antibiotic
drug resistance
resistance gene
